FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689869
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27440.39775667554274796No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24680.35774907989777766No Hit
GATCTATGGTGTGTCCAATTTACAAACTGGGGTCCCAACAAGGTTCAGTG21720.31484238311911394No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAACTCAGGCC20180.292519304389674No Hit
CCATAGATCAGGACGTGAGGGGCTTTCCCTGGTTTCTGTTGATACCAATT17730.2570053155019286No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16350.2370015176794435No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16230.2352620569992274No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG15810.22917394461847104No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14940.21656285468690434No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13790.19989302316816673No Hit
GTTACAACACCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAA13500.19568932652431112No Hit
GAGTTACAACACCCCGCTCACTTTCGGCGGAGGGACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCAT13190.19119571976708621No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGTTCGCCCGTCACAAA12940.18757184334996935No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11900.17249651745476316No Hit
GTTGTAACTCTGCTGACAATAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGCGTGAACT11230.16278452865688994No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11110.16104506797667384No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11060.16032029269325046No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10920.158290921899665No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10740.15568173087934087No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10570.15321749491570139No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10520.15249271963227803No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10160.14727433759162972No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10100.14640460725152166No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9970.1445201915146209No Hit
GTGTTGTAACTCTGCTGACAATAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGCGTGAACT9950.14423028140125155No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGTT9470.13727243868038716No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9470.13727243868038716No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9440.13683757351033313No Hit
GAGTTCACGCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTATTGTCAGCAGAGTTACAACACC9360.13567793305685572No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATCAGCAACTTTTTAAATTGGTATCAACAGAAA9260.13422838249000899No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTATTGTCAGCAG9190.13321369709321623No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8940.12958982067609937No Hit
TCTATGGTGTGTCCAATTTACAAACTGGGGTCCCAACAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCACG8900.12901000044936067No Hit
GTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCCCTCCGCCGAAAGTG8670.12567603414561315No Hit
CTGCTACTCTGGCTCCGAGGCGCCCGATGTGACATCCAGATGACCCAGTC8500.12321179818197368No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGTGTTGTAACTCTGCTGACA8480.12292188806860432No Hit
GTCTCCATCTTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATCAGCAA8470.12277693301191966No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8100.1174135959145867No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCAACCTTGGTCC7960.11538422512100124No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGCGCCCGATGTGACAT7830.11349980938410045No Hit
GTCCCAGATCCACTGCCACTGAACCTTGTTGGGACCCCAGTTTGTAAATT7580.10987593296698359No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7420.10755665206002879No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7370.1068318767766054No Hit
CCATCTTCCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCG7300.10581719137981269No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7260.10523737115307398No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7260.10523737115307398No Hit
CTGCTGATGGTGAGCGTGAACTCTGTCCCAGATCCACTGCCACTGAACCT7240.10494746103970463No Hit
ATCTGGGACAGAGTTCACGCTCACCATCAGCAGTCTGCAACCTGAAGATT7200.10436764081296593No Hit
GTTCAGTGGCAGTGGATCTGGGACAGAGTTCACGCTCACCATCAGCAGTC7060.10233827001938048No Hit
GACCAAGGTTGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT7020.1017584497926418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTT200.006650677453.08905850
TACGTAT400.00845315149.9594470
GTGGTAT2100.035.203591
TAGTCAC807.439121E-834.31019238
GAATACG1005.0749804E-1033.581519
ATACGTA1005.0749804E-1033.5815111
ACGTACC1058.56744E-1031.98470913
AATACGT1058.56744E-1031.9823910
TACGTAC1058.56744E-1031.9823912
CGGCAAA1302.9471401E-530.74426870
CGTACCA1101.4097168E-930.53307214
ATCAACG23300.029.976173
TCAACGC23700.029.328564
CAACGCA23850.029.2848975
AACGCAG24250.028.9424156
GTAAAAC1053.0284355E-828.7820643
AATAGTC953.4737604E-728.78195236
CTAGAGA1302.7102942E-1028.4130634
GTTCACG12850.027.99283470
ACGCAGA25350.027.8197
Produced by FastQC (version 0.11.5)