FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820490
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGC28460.34686589720776606No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGGTGACTCAGCCGCTCTCAGTGT25600.31200867774134966No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24650.3004302307157918No Hit
CCTATGAGGTGACTCAGCCGCTCTCAGTGTCAGTGGCCCTGGGACAGACG23840.2905580811466319No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCAC23670.28848614852100574No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG23480.28617045911589417No Hit
GGATTACCTGTGGGGGCAATGACATTGGAGATAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC21740.2649636193006618No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21150.2577727943058416No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19990.24363490109568672No Hit
CCCCTGTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCT18600.22669380492144936No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCACAGAGGCAGTG17450.2126777901010372No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17450.2126777901010372No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17290.21072773586515375No Hit
GACTATTACTGTCAAATATGGTACGTCAACACTGCCTCTGTGCTTTTCGG16950.20658387061390143No Hit
CCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC16560.2018306134139356No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15910.19390851808065912No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15800.19256785579348926No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTC15680.1911053151165767No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15610.1902521663883777No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGGTGACT15360.1872052066448098No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14830.18074565198844594No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTATCT14570.17757681385513535No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14210.17318919182439763No Hit
TTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCTGACCGATTCTCTGGCTCCAACTCGGGGAATATGGCCACC13860.1689234481834026No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTAGTGATGGTCAGGGTGG13760.16770466428597544No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12920.15746687954758742No Hit
GTGTTGACGTACCATATTTGACAGTAATAGTCAGCCTCATCCCCGGCTTG12830.156369974039903No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12820.15624809565016026No Hit
CTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12310.1500322977732818No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12020.1464978244707431No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11400.13894136430669476No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGG11160.13601628295286963No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11150.1358944045631269No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11110.13540689100415604No Hit
GTCTCTGGTGGCTCCATCAGTCCTTACTACTGGAGCTGGATCCGGCAGCC10720.13065363380419018No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTGGGGGCAATGACATTGGAGAT10650.12980048507599118No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGGTGACTCAGCCGCTCTCAGTGT10550.12858170117856402No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG10130.12346280880937001No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10090.12297529525039914No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT10080.12285341686065643No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10000.12187838974271471No Hit
GGCCAGGCCCCTGTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGG9950.12126899779400113No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9510.1159063486453217No Hit
GTATCAACGCAGAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCT9480.11554071347609354No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCACAGAGGCAGTGT9470.11541883508635084No Hit
GTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGC9330.11371253762995283No Hit
CGTCAACACTGCCTCTGTGCTTTTCGGCGGAGGGACCAGGCTGACCGTCC9290.11322502407098198No Hit
GGGTGGCCATATTCCCCGAGTTGGAGCCAGAGAATCGGTCAGGGATCCCA9260.11285938890175384No Hit
GTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCTGACCG9240.1126156321222684No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGG9060.11042182110689953No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9020.10993430754792867No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGG9010.10981242915818595No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC8930.10883740204024424No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8660.10554668551719096No Hit
TCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAATATGG8570.10444978000950653No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA8500.1035966312813075No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8470.10323099611207938No Hit
GACCATCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAA8460.10310911772233665No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8340.10164657704542408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)