FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055929
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT79570.7535544529982603No Hit
CTATAATATTGGTGACAATAATAAACTGCCACATCCTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC53720.5087463266943137No Hit
CTCTTATACACCTCCGACAACAGGAACTACTTGGCTTGGTTGCAGCAGAA49250.4664139350278286No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39930.37815042488652173No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39930.37815042488652173No Hit
CTTATACACCTCCGACAACAGGAACTACTTGGCTTGGTTGCAGCAGAAAC29060.27520789750068425No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27920.2644117170756746No Hit
GTATAAGAGACTCCGGCTGGACTTGCAGTTGATGGTGGCCCGCTCGCCCAGAGACACAGCCAGAGAGTCTGGAGA27360.2591083302002313No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26250.24859625978640607No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23740.2248257221839726No Hit
CCTTAGGCGGCTGTCCTGGTTTCTGCTGCAACCAAGCCAAGTAGTTCCTG23540.22293165544274282No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23080.2185753019379144No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22460.21270369504010214No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22140.20967318825413453No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22070.2090102648947041No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21830.20673738480522838No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21230.2010551845815391No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20750.1965094244025877No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19300.1827774405286719No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19090.18078867045038066No Hit
GTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATA18130.1716971500924778No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17800.1685719399694487No Hit
GTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATACACCTCCGACAACAGGAACTA17280.1636473664422513No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT17280.1636473664422513No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17090.16184800303808305No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16660.15777575954443906No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15470.146506062434122No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTAAAGCCGGGGGGGTCCCTTAGACTCTCCTGTGCAGC15230.14423318234464627No Hit
AACCAGGACAGCCGCCTAAGGTGCTCATTTATTGGGCATCCACCCGGGAA15070.14271792895166246No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15010.14214970892929354No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14910.14120267555867866No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14900.1411079722216172No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14770.13987682883981783No Hit
CTCTGGCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGC14630.138550982120957No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAATGTCTGG14220.134668145301436No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC13860.13125882516722243No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC13840.13106941849309944No Hit
CTCTCTGGCTGTGTCTCTGGGCGAGCGGGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATACACCTCCGA13840.13106941849309944No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAATGTCTGGATGAGGTGGGTCCGCCAGGCTCCAGGGAAGGGG13820.13088001181897646No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13660.12936475842599265No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13370.1266183616512095No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13230.12529251493234866No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13230.12529251493234866No Hit
CATTTATTGGGCATCCACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA13180.12481899824704124No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13050.12358785486524188No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12980.12292493150581148No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC12870.1218831947981351No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12850.12169378812401212No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT12790.12112556810164318No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12350.11695862127093773No Hit
GTAATAGACGCCTGTGTCCTCGGTTGTCAGGCTGTTCATTTGCAGAAAAA12140.11496985119264648No Hit
GTCCTGGTTTCTGCTGCAACCAAGCCAAGTAGTTCCTGTTGTCGGAGGTG12070.11430692783321605No Hit
ATAGTGCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA11980.11345459779966266No Hit
GTCAGGGACCCCGGATTCCCGGGTGGATGCCCAATAAATGAGCACCTTAGGCGGCTGTCCTGGTTTCTGCTGCAA11970.11335989446260117No Hit
GGCCACCATCAACTGCAAGTCCAGCCGGAGTCTCTTATACACCTCCGACA11880.11250756442904779No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTATTGTCACCAATATTATAGTGCT11870.1124128610919863No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11760.11137112438430992No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11730.11108701437312547No Hit
CGCCTAAGGTGCTCATTTATTGGGCATCCACCCGGGAATCCGGGGTCCCT11500.10890883762071123No Hit
GTGTATAAGAGACTCCGGCTGGACTTGCAGTTGATGGTGGCCCGCTCGCC11310.10710947421654296No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11270.10673066086829702No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA11150.10559422082355915No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11150.10559422082355915No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10990.10407896743057535No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10920.10341604407114495No Hit
GGCTGAGGATGTGGCAGTTTATTATTGTCACCAATATTATAGTGCTCCGT10890.10313193405996046No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10850.10275312071171452No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10800.10227960402640708No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10690.1012378673187307No Hit
GGATCTCTGGTGCCTACGGGGACGTCGTGATGACCCAGTCTCCAGACTCTCTGGCTGTGTCTCTGGGCGAGCGGG10590.10029083394811583No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)