FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885238
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCAACTGATGGCCAGGGAGG36970.41762780178889747No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGG36200.40892957600103025No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC28470.321608426208545No Hit
GTATATGGTGGTACTACTACCAGTAATGTATGAAATCCACTCCAGCCCCTTCCCCGGAGCCTGGCGGACCCAGTT28430.321156570323461No Hit
ACCATATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAAAACTCACTGTATCTA27880.3149435519035559No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT25760.2909951899941033No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC23820.2690801795675287No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA23290.2630930890901656No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23220.26230234129126856No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT23130.2612856655498295No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22390.25292633167577533No Hit
ATAATACACAGCCGTGTCCTCGTCTCTCAGTCTGTTCATTTGTAGATACA21160.23903176320944197No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20850.2355298801000409No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC20260.22886500579505173No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGGC20150.22762240211107068No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGG19620.22163531163370753No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19540.22073159986353955No Hit
CAATAATACACAGCCGTGTCCTCGTCTCTCAGTCTGTTCATTTGTAGATA17170.19395913867231185No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTGAC17160.19384617470104085No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC16410.18537387685571566No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG15520.1753200834125964No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15110.1706885605904853No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC14790.16707371350981318No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14580.16470147011312214No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGGCTGTCATCCCATG14450.1632329384865991No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14420.16289404657278608No Hit
GTGTATTATTGTGCGAAAGATCTCCTCATGCGGATGGACGTCTGGGGCAA14160.15995698331974No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13880.15679399212415193No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13740.15521249652635788No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTCCGTAGCTTTAGTATGAACTGGGTCCGCCAGGCTCCGGGGAAGGGG13610.15374396489983483No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13430.1517106134169568No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13170.14877355016391072No Hit
GTATTATTGTGCGAAAGATCTCCTCATGCGGATGGACGTCTGGGGCAAGG13100.1479828023650137No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13020.14707909059484567No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT12960.14640130676721966No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC12740.14391609939925762No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12500.14120496408875355No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGACTTGGGGCTGTGCTGGG12360.1396234684909595No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12310.1390586486346045No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTTGGCTCCAGT12140.13713826112299743No Hit
CCCCTGAGGGCCGCTGATTTTTACTATCGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGGTACCAGTTTA12020.1357826934677454No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA11620.13126413461690528No Hit
CTCCAGAGACAATGCCAAAAACTCACTGTATCTACAAATGAACAGACTGA11470.12956967504784023No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11340.1281011434213172No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG11240.12697150370860716No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11210.12663261179479418No Hit
CTCTTGTTCTGGAGGCAGCTCCAACATCGGAAGTTATACTGTAAACTGGTACCAGCAAGTCCCAGGAACGGCCCC11120.12561593605335514No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC11020.12448629634064512No Hit
GCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGACCAAGGT10990.1241474044268321No Hit
GGCAGAGGGTGACCATCTCTTGTTCTGGAGGCAGCTCCAACATCGGAAGT10570.11940291763344998No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10560.11928995366217898No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGAC10440.11793438600692695No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10430.11782142203565595No Hit
TCCTCAGACTGGAGCCAACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC10410.11759549409311393No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10290.11623992643786189No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10270.1160139984953199No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10250.1157880705527779No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10120.11431953892625486No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCCTTG9890.11172136758702181No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9780.11047876390304076No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9760.11025283596049877No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9730.10991394404668575No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA9660.10912319624778874No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9600.10844541242016272No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9430.10652502490855567No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT9420.10641206093728467No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9290.10494352931076163No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9270.10471760136821961No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9190.1038138895980516No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9180.1037009256267806No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG9090.10268424988534157No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC8990.10155461017263155No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC8950.10110275428754753No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8900.10053793443119252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG150.002444092268.3595314
AGGGGGG2800.061.10661770
TAGTCTA252.6871153E-454.6907118
TATCACG200.007634840451.269642
ACGTAGT200.007634840451.2696415
GGTATCA8650.044.678781
TGGTATC2350.043.633742
CGTATGC2200.042.42476341
GTGGTAT2450.041.8787881
TCGTATG2250.041.42519440
ATGCCGT2400.040.48698844
TCTCGTA2050.040.32969338
GACTAGG2500.038.30513426
ATCTCGT2100.037.67973337
CACGAGA2550.037.5328421
GCCGTCT2550.036.8374546
CGAGACT2600.036.8131323
TCTCCGA2600.036.8110511
TCTTATA655.862257E-736.8089752
GTATGCC2550.036.6499142
Produced by FastQC (version 0.11.5)