FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885238
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGG35510.401135061983331No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCAACTGATGGCCAGGGAGG35160.3971813229888459No Hit
GTATATGGTGGTACTACTACCAGTAATGTATGAAATCCACTCCAGCCCCTTCCCCGGAGCCTGGCGGACCCAGTT27240.3077138577422117No Hit
ACCATATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAAAACTCACTGTATCTA27060.30568050625933363No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26730.30195269520739054No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC26030.29404521721842036No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC24120.27246909870565883No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA23390.2642227288028756No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT23380.2641097648316046No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT21040.23767619555418992No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG20260.22886500579505173No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC20050.2264927623983607No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19960.22547608665692162No Hit
ATAATACACAGCCGTGTCCTCGTCTCTCAGTCTGTTCATTTGTAGATACA19540.22073159986353955No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18040.20378700417288909No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGGC17840.20152772474746902No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17550.19825176958060994No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGG17360.19610545412646088No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC16260.1836794172866506No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTGAC15620.1764497231253064No Hit
CAATAATACACAGCCGTGTCCTCGTCTCTCAGTCTGTTCATTTGTAGATA15350.17339969590098933No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTCCGTAGCTTTAGTATGAACTGGGTCCGCCAGGCTCCGGGGAAGGGG14330.16187737083134707No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14260.16108662303245003No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGGCTGTCATCCCATG14240.16086069508990802No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13810.15600324432525492No Hit
GTGTATTATTGTGCGAAAGATCTCCTCATGCGGATGGACGTCTGGGGCAA13480.1522754332733118No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13390.1512587575318728No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13390.1512587575318728No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13350.15080690164678878No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC13150.14854762222136872No Hit
GTATTATTGTGCGAAAGATCTCCTCATGCGGATGGACGTCTGGGGCAAGG12830.1449327751406966No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12320.1391716126058755No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12290.13883272069206248No Hit
CCCCTGAGGGCCGCTGATTTTTACTATCGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGGTACCAGTTTA12150.13725122509426843No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12030.1358956574390164No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12010.13566972949647438No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC11820.13352341404232534No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11730.13250673830088633No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGACTTGGGGCTGTGCTGGG11490.12979560299038226No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11420.12900485519148525No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG11390.1286659632776722No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC11370.1284400353351302No Hit
CTCCAGAGACAATGCCAAAAACTCACTGTATCTACAAATGAACAGACTGA11350.1282141073925882No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT11300.12764928753623317No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11010.12437333236937412No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11000.1242603683981031No Hit
CTCTTGTTCTGGAGGCAGCTCCAACATCGGAAGTTATACTGTAAACTGGTACCAGCAAGTCCCAGGAACGGCCCC10550.11917698969090798No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10520.11883809777709496No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTTGGCTCCAGT10450.11804734997819795No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10280.1161269624665909No Hit
GCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGACCAAGGT10270.1160139984953199No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9960.11251211538591882No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA9880.1116084036157508No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGAC9850.11126951170193779No Hit
TCCTCAGACTGGAGCCAACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC9740.11002690801795675No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCCTTG9300.10505649328203263No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9070.10245832194279957No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9050.10223239400025756No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8930.10087682634500553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)