FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886774
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCTGTTTGGTGACTCACCCCCGGTAAAGGTGACGTTCG56010.6316152706326527No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39080.44069853198221864No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36550.4121681510734415No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA28440.3207130565397722No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCT27290.3077447015812371No Hit
GTGTATTACTGTCAGCTGTTTGGTGACTCACCCCCGGTAAAGGTGACGTT27190.3066170185413645No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT25490.28744640686353007No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23020.2595926357786764No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22920.25846495273880377No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21670.24436891474039607No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20770.23421976738154252No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20350.22948349861407752No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19260.217191753479466No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18940.21358316775187364No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18820.2122299481040265No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18630.2100873503282685No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA18530.2089596672883959No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18460.20817028916048508No Hit
GTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCTGA17490.19723176367372072No Hit
CTCCTGCAGGGCCAGTCTGACTGTTAGCAACAACTACTTAGCCTGGTATC17460.1968934587617589No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT17430.19655515384979713No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15300.17253550510051038No Hit
GTGATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAGGTTCAGGGAGA15250.17197166358057409No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15070.16994183410880337No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGATACCAGGCTAAGTAGTTGTTGCTAACAGTCAGA14930.16836307785298169No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14710.16588217516526194No Hit
CTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGC14510.16362680908551672No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT14500.16351404078152945No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14340.16170974791773327No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14100.15900330862203899No Hit
GTCTGACTGTTAGCAACAACTACTTAGCCTGGTATCAGCACAAACCTGGC14090.15889054031805172No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCTGACTGTTAGCAA14080.15877777201406446No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13910.15686071084628103No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13860.1562968693263447No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC13860.1562968693263447No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13320.15020738091103256No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCTGTTTGGTGACTCA13150.14829031974324913No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC13030.14693710009540198No Hit
GGTTTGTGCTGATACCAGGCTAAGTAGTTGTTGCTAACAGTCAGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC12860.14502003892761853No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT12770.14400512419173317No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA12680.14299020945584784No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT12640.14253913623989878No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12250.13814117238439558No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12040.13577303800066307No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11820.13329213531294332No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGTTGGATCCGGCAGCC11750.1325027571850325No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11540.13013462280129998No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT11530.13002185449731274No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11370.12821756163351655No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11210.12641326876972034No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10680.1204365486583955No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCTCCGGAGAAAATTTGCTGACGCAGTCTC10490.11829395088263751No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10410.11739180445073942No Hit
CCTTTACCGGGGGTGAGTCACCAAACAGCTGACAGTAATACACTGCAAAA10380.11705349953877763No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10330.11648965801884133No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10210.11513643837099419No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC10060.11344491381118527No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10050.113332145507198No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10010.11288107229124894No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT9950.11220446246732539No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9910.11175338925137634No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9720.10961079147561836No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9650.10882141334770754No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9520.10735542539587312No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9460.10667881557194957No Hit
ATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGTCCACCT9320.10510005931612791No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9270.10453621779619159No Hit
AACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCC9250.10431068118821707No Hit
CTGCTACTCTGGCTCCCAGATACCTCCGGAGAAAATTTGCTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTC9210.10385960797226802No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC9110.10273192493239541No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC9020.10171701019651004No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8990.10137870528454827No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8950.10092763206859923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT309.279882E-657.33609437
AATATCG352.3228125E-549.1033336
TCGTATG4600.048.80535540
CTTATAC4650.048.4342041
GCTAATC4050.048.25520733
CGTATGC4800.047.5456741
TTATACA4900.046.633172
ATGCCGT4850.046.49139844
TATACAC5100.046.141863
ACGAGAC4850.045.72558622
ATCTCGT4250.045.3292437
CACGAGA5000.045.03364621
TAATGAT4700.044.3182770
CTAATCT4350.044.1691134
ATCTCCG5100.044.13320510
GTATGCC5200.043.93855342
AATCTCG4400.043.74660536
TAATCTC4450.043.21331435
CCGTCTT5200.042.8597647
TACACAT5350.042.7107545
Produced by FastQC (version 0.11.5)