FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155137
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCGACAGTAAGAACTACTTAGCTTGGTTCCAGCAGAA53590.46392765533438896No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45420.39320011392588067No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCT36550.31641268524858956No Hit
ATATTATAGCAGTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT36080.3123439037966925No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36030.31191105470606517No Hit
GCTATAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAAT34960.30264808416663996No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29400.25451526528887913No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28650.24802252892946897No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28360.24551200420383038No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGAACCAAGCTAAGTAGTTCTTACTGTCGGAGCTGTATAAAACACT27550.2384998489356674No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25000.21642454531367272No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23980.2075944238648749No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23460.2030927933223505No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23410.20265994423172315No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA22190.19209842642041594No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21710.18794307515039343No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20830.18032493115535214No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20120.17417847406844383No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19910.172360507887809No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19670.17028283225279772No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19420.168118586799661No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18850.16318410716650925No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18820.16292439771213285No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18750.16231840898525457No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18120.15686451044335No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17870.15470026499021328No Hit
GTAATACACAGCCGTGTCTTCGGCTCTCAGATTGTTCATTTGCAGAAACA17790.15400770644520953No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17560.15201660062832376No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17530.15175689117394733No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16900.14630299263204277No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16890.1462164228139173No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC16320.14128194318076556No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16280.1409356639082637No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15970.13825199954637415No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA15940.13799229009199776No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15040.13020100646070554No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14330.12405454937379722No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14050.12163059446628408No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTGCCGGGT13950.1207648962850294No Hit
GTGTATTACTGTACAAGGTCGGGTGGGAGCGACTCCCGATTTGACCACTGGGGCCAGGGAACCCTGGTCACCGT13910.12041861701252751No Hit
GATTTCACTCTCACCATCGGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTATTGTCAGCAATATTATAGCAGT13850.11989919810377471No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGCAA13720.1187737904681436No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13350.11557070719750125No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13270.1148781486524975No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCGA13130.11366617119874094No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG12980.1123676239268589No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12890.11158849556372967No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCGGCAGCC12790.11072279738247497No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12500.10821227265683636No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12480.10803913302058545No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11930.10327779302368464No Hit
TCTTCAGCCTGCAGGCTGCCGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC11860.10267180429680636No Hit
GCTCATTTACTGGGCATCTACCCGGCAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT11790.10206581556992808No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11670.10102697775242243No Hit
GTCAGGGACCCCGGATTGCCGGGTAGATGCCCAGTAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGAA11670.10102697775242243No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11640.10076726829804604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAA200.00780221550.99014724
CGATAGG200.00783838750.93052316
CGATTGT652.025242E-550.25161770
TGGTATC2500.050.2340432
GTGGTAT2600.048.331261
GGTATCA9650.046.456491
GTATCAA26800.037.0039251
ATCAACG30950.032.2365043
TCAACGC31300.031.9844514
CAACGCA31900.031.4906085
AACGCAG32200.031.199926
ACGCAGA32900.030.5360937
CGCAGAG32750.030.468688
CGTAAGT450.004865027530.3479731
ACGGGTA1800.030.18105116
TATCAAC33050.030.090722
AGAGTAC34350.028.95066311
GCAGAGT34850.028.632699
CAGAGTA34900.028.59167110
TACTCGA655.9251714E-428.31711666
Produced by FastQC (version 0.11.5)