FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004684476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291287
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCGTCAGAGTGGAGCCCACTGATGGCCAGGGAGG68520.5306333913374796No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCAGGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTC66390.5141382202407366No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACTC40840.31627360919764547No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38100.2950544689135723No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37130.2875425834845391No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT32030.24804710339374594No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC31040.24038033372906253No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30620.237127764780409No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29380.22752494217009853No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28100.21761235108848767No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28040.2171476983815372No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC27160.21033279201292973No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26160.20258858023042128No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25710.19910368492829247No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24430.18919109384668165No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACTCCCGGGCAGAGGGTCAC23560.18245362959589928No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23540.18229874536024912No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC23300.1804401345324471No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG23300.1804401345324471No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23150.17927850276507082No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACCTGACCATTC22380.1733154596925393No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22150.17153429098256237No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG21900.16959823803693524No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT21800.16882381685868442No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21650.16766218509130812No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21650.16766218509130812No Hit
GAAGTAATACTGTAAGCTGGTATCAGCAGCTCCCAGGAACGGCCCCCAGA21320.16510659520308033No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21250.16456450037830475No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG20630.15976308907314948No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20240.15674284647797118No Hit
CTCCTGTACAGCCTCTGGATTCACCTTCAGTAACAATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG19900.15410981447191832No Hit
GCATGGGATGACAGCCTGAATGGTCAGGTGGTGTTCGGCGGAGGGACCAA19090.14783700292808646No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19070.1476821186924363No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19020.1472949081033109No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA18870.14613327633593462No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT18820.1457460657468092No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT18680.144661876097258No Hit
CCCCTGAGGGCCGCTGATTATTATTGTAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGCTTA18550.14365512856553192No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18010.13947325420297735No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17800.13784696972865057No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17680.13691766431474955No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT17610.13637556948997398No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17440.13505905348694752No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGCTTACAGTATTACTT17350.13436207442652176No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC17290.13389742171957125No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16600.1285539155896404No Hit
GTTCTAAGGCTGTTCATCTGCAGATACAGTGTGTTCTTGGAATTGTCTCT16550.128166705000515No Hit
GTAGAACACAGCAGTGTCCTCAGTTCTAAGGCTGTTCATCTGCAGATACA16290.1261532099370628No Hit
CCACCTGACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC15780.12220366192798347No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15620.12096458804278212No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG15600.12080970380713195No Hit
GTCAGGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCC15280.11833155603672924No Hit
CCCTCAGCGTCTGGGACTCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG14990.11608573461980179No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14980.11600829250197672No Hit
GCCTTAGAACTGAGGACACTGCTGTGTTCTACTGTGCGAGAAGCCCTACG14890.11531131344155095No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAGCTGGTATCAGCAGCTCCCAGGAACGGCCCC14770.11438200802764992No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC14690.11376247108504926No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC14670.1136075868493991No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCGTCAGAGTGGAGCCCAC14640.11337526049592384No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14620.11322037626027366No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14560.11275572355332315No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC14510.11236851296419774No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14470.1120587444928974No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14460.11198130237507231No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC14320.11089711272552112No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14260.11043246001857061No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14220.11012269154727027No Hit
GATTATTATTGTAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGA13870.10741221742339231No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13860.10733477530556725No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTTCAGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCACT13840.10717989106991707No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA13720.10625058565601606No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13550.10493406965298963No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA13430.10400476423908861No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13330.10323034306083775No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT13180.1020687112934615No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC13170.1019912691756364No Hit
GCTGATACCAGCTTACAGTATTACTTCCGATGTTGGAGCTGCTTCCAGAA13090.10137173223303572No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12950.10028754258348453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)