FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291287
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCAGGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTC60450.4681376022526363No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACTC44710.3462437087959532No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42150.3264185266327315No Hit
GTAATAATCAGCCTCATCGTCAGAGTGGAGCCCACTGATGGCCAGGGAGG36870.2855290884210869No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35920.27817208722770387No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT34920.27042787544519536No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34560.2676399592034923No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29070.22512423651752086No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28980.22442725745709513No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACTCCCGGGCAGAGGGTCAC28130.21784467744196293No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC27600.21374024519723345No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27290.21133953954465584No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27040.20940348659902872No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG25850.20018787457784368No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC25710.19910368492829247No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25660.19871647433916703No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24940.19314064185576096No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC23780.18415735618805115No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23300.1804401345324471No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22450.1738575545173149No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21960.17006289074388575No Hit
GAAGTAATACTGTAAGCTGGTATCAGCAGCTCCCAGGAACGGCCCCCAGA21450.16611334273480644No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT21180.16402240555352915No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20990.16255100531485253No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20660.15999541542662477No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20550.15914355213054884No Hit
CTCCTGTACAGCCTCTGGATTCACCTTCAGTAACAATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG20160.1561233095353705No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA19560.15147678246586546No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT19140.14822421351721188No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19110.14799188716373665No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19090.14783700292808646No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18770.14535885515768376No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18620.1441972233903075No Hit
CCCCTGAGGGCCGCTGATTATTATTGTAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGCTTA18380.14233861256250546No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC18340.14202884409120511No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18000.13939581208515225No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACCTGACCATTC17950.13900860149602684No Hit
GCATGGGATGACAGCCTGAATGGTCAGGTGGTGTTCGGCGGAGGGACCAA17700.13707254855039971No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAGCTGGTATCAGCAGCTCCCAGGAACGGCCCC17670.13684022219692446No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGATACCAGCTTACAGTATTACTT17570.13606580101867363No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17530.13575603254737328No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17500.13552370619389803No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17160.13289067418784514No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT16770.12987043159266684No Hit
GTTCTAAGGCTGTTCATCTGCAGATACAGTGTGTTCTTGGAATTGTCTCT16710.12940577888571633No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16280.1260757678192377No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15830.12259087251710889No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC15700.1215841249853828No Hit
GTCAGGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCC15630.12104203016060722No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15550.12042249321800653No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTTCAGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCACT15500.12003528262888111No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15370.11902853509715501No Hit
GTAGAACACAGCAGTGTCCTCAGTTCTAAGGCTGTTCATCTGCAGATACA15350.11887365086150484No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15270.11825411391890417No Hit
CCCTCAGCGTCTGGGACTCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG15150.11732480850500315No Hit
GCCTTAGAACTGAGGACACTGCTGTGTTCTACTGTGCGAGAAGCCCTACG14840.11492410285242553No Hit
ATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTTCAGG14360.11120688119682146No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC14210.1100452494294452No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14200.10996780731162012No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14200.10996780731162012No Hit
GCCCCCAGACTCCTCATCTACAATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTTCAGGT13920.10779942801251775No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA13900.10764454377686758No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13840.10717989106991707No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13830.10710244895209198No Hit
GTCTGGGACTCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAG13800.10687012259861672No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG13460.10423709059256385No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT13420.1039273221212635No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA13390.10369499576778826No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13330.10323034306083775No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC12980.1005198689369598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4150.043.627931
GGTATAC350.001421838339.0023888
GTATCAA36000.037.862321
GACAGGT2150.034.9559481
TTCACGG5850.033.6418870
TATCAAC41150.033.0891462
ATCAACG43250.031.5577413
TCAACGC43650.031.3455074
AACGCAG43900.031.0952996
CAACGCA44300.030.9662045
TCGTTAA900.00591564929.15629270
CGCAGAG47050.028.9409338
AGAGTAC47500.028.73860511
CAGAGTA48300.028.26260210
GCAGAGT49000.027.7892049
GCCAGAG19050.027.20488770
ATAGCGG1356.553819E-926.44379663
ACGAACC1508.54925E-1025.73724752
ACGCAGA53250.025.6994637
AATCGTT1501.031367E-925.32470139
Produced by FastQC (version 0.11.5)