FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719836
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22810.3168777332614651No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19680.27339560677709923No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18200.2528353680560572No Hit
GATCTATAGTGCATCCACTTTGCAAAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA15730.21852199667702085No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15630.21713279135803154No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14580.20254613550864364No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13640.18948760551014396No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12680.17615123444784644No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12510.1737895854055646No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11940.16587111508732544No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11700.16253702232175105No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11320.15725804210959163No Hit
GTTGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGAGCATTAACAACTATTTAAATTGGTATCAGCAGAAA11210.15572991625870336No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11080.15392394934401726No Hit
GTAATATACGGCTGTGTCCTCGCTTTTCAGGCTGTTCATTTCCAGATACA11030.15322934668452257No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10210.14183786306881013No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10160.14114326040931546No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10120.14058757828171975No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10110.1404486577498208No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10040.13947621402652827No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGAGCATTAACAA9990.1387816113670336No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9770.1357253596652571No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9720.13503075700576242No Hit
GAGTTATGATACGTTCATGTTCACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCAT9580.13308586955917737No Hit
CTATAGATCAGGAGTTTAGGGGCTTTACCTGGTTTCTGCTGATACCAATT9470.1315577437082891No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9390.13044637945309764No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9330.12961285626170405No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9250.1285014920065126No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9050.12572308136853394No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8910.12377819392194889No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8820.12252790913485849No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8800.12225006807106062No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC8760.12169438594346489No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8710.12099978328397024No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8650.12016626009257664No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8570.11905489583738518No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8440.11724892892269906No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC8430.11711000839080012No Hit
ACTTACTACTGTCAACAGAGTTATGATACGTTCATGTTCACTTTTGGCCA8330.1157208030718108No Hit
GTTATGATACGTTCATGTTCACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCAT8330.1157208030718108No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8310.11544296200801293No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8300.115304041476114No Hit
TATCATAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT8220.11419267722092255No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8050.11183102817864068No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8040.11169210764674176No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8000.11113642551914603No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7970.11071966392344923No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA7860.10919153807256096No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7730.10738557115787485No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7590.1054406837112898No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7390.10266227307331115No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7340.10196767041381648No Hit
GTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT7320.10168982935001862No Hit
GTATATTACTGTACCACAGGGTCGATCTTTGGAGTGATTACGTCTGCCGA7280.1011341472224229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2200.046.4966551
GTATCAA18350.036.6060831
TCAACGG400.002748471234.09991519
AGTTTAT3150.032.446782
TATCAAC21050.031.236772
ATCAACG22050.029.6635683
TCAACGC22400.029.2000754
AACGCAG22650.028.7373566
CAACGCA22800.028.6917785
AGAGTAC23800.027.49197411
ACGCAGA23700.027.464187
GGAGTCG500.00799058127.41527632
CGCAGAG23800.027.3487858
GCTTGTA500.008188656527.2780361
CTTAATA500.0082248327.2534053
GCAGAGT24400.026.6762739
ACGGGCC1351.0690201E-825.25217416
GGTATCA15350.024.8789921
CAGAGTA26350.024.83145910
AATGGTA2101.2968849E-624.74550670
Produced by FastQC (version 0.11.5)