FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908265
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGCTTACTACTGGAGCTGGATCCGCCAGTC41050.45196060621074247No Hit
CAGTAATATGTGCCCGTGTCCGCGGCGGTCACAGAGCTCAGTCCCAGGGAGAACTGGTTCTTAGACGTGTCGA29170.3211617754730172No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23090.25422095974192555No Hit
GTATTACTGTCATTGTCAGCAGTATGGTGCCTTGCCTAAGACGTTCGGCC22330.24585335777553904No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21580.2375958558350261No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT17700.19487704579610576No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16640.1832064430535141No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA16560.18232564284652608No Hit
GTATGGTGCCTTGCCTAAGACGTTCGGCCAAGGTACCAGGGTTGATATGAAACGAACTGTGGCTGCACCAT16450.1811145425619175No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14700.16184703803405392No Hit
ATATTACTGTGCGCGAGGCAGACCGACGTATAACGATTTAGCGGGCTACTACTACTACGGTATGGACGTCTGGGG14620.1609662378270659No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14390.15843393723197524No Hit
CATATTACTGTGCGCGAGGCAGACCGACGTATAACGATTTAGCGGGCTAC14270.15711273692149316No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14150.15579153661101108No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13840.1523784358089324No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13730.15116733552432385No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13530.14896533500685372No Hit
GCCTAAGACGTTCGGCCAAGGTACCAGGGTTGATATGAAACGAACTGTGG13080.14401083384254595No Hit
GTACCAGGGTTGATATGAAACGAACTGTGGCTGCACCATCTGTCTTCATC13040.14357043373905193No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12760.14048763301459374No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12390.13641393205727403No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACGCAGTGCCA12260.13498263172091846No Hit
GTAATATGTGCCCGTGTCCGCGGCGGTCACAGAGCTCAGTCCCAGGGAGA12230.13465233164329793No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12170.1339917314880569No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12070.13289073122932185No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC11900.13101903078947225No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11850.13046853066010472No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11800.12991803053073717No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11730.12914733034962264No Hit
CCCTGGGACTGAGCTCTGTGACCGCCGCGGACACGGGCACATATTACTGT11640.1281564301167611No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11250.12386252910769435No Hit
CTCCTGCAGGGCCAGTCAGCGTGTTGACAGCACCTACTTGACCTGGTTCC11200.1233120289783268No Hit
GTCTTAGGCAAGGCACCATACTGCTGACAATGACAGTAATACACTGCAAA11110.12232112874546526No Hit
CATCTATGGTGCCTCCAGCAGGGCCACTGGCGTCCCAGCCAGGTTCAGTGGCACTGCGTCTGGGACAGACTTCA11030.1214403285384772No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10980.12088982840910967No Hit
GTGTATTACTGTCATTGTCAGCAGTATGGTGCCTTGCCTAAGACGTTCGGCCAAGGTACCAGGGTTGATATGAAA10950.12055952833148915No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGCGTGTTGACAG10920.12022922825386864No Hit
GTCCCAGGGAGAACTGGTTCTTAGACGTGTCGACTGATATGGTGACTCGA10570.11637572734829592No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10490.11549492714130788No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAACAGTCCTGCGCCCCACTGCTGTAACTGCAC10410.11461412693431984No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10330.11373332672733177No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10300.11340302664971126No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10280.11318282659796425No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10150.11175152626160868No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10110.11131112615811464No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10080.11098082608049413No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9930.10932932569239155No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGCGTGTTGACAGCA9820.10811822540778297No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9630.10602632491618635No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9590.10558592481269233No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9470.10426472450221025No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9390.10338392429522221No Hit
GCACCATACTGCTGACAATGACAGTAATACACTGCAAAATCTTCAGGCTC9380.10327382426934871No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9350.10294352419172817No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9340.10283342416585468No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG9320.10261322411410767No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9240.10173242390711962No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC9100.10019102354489054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)