FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908265
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGCTTACTACTGGAGCTGGATCCGCCAGTC39830.4385284030541747No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34920.3844692903502832No Hit
CAGTAATATGTGCCCGTGTCCGCGGCGGTCACAGAGCTCAGTCCCAGGGAGAACTGGTTCTTAGACGTGTCGA29680.32677687679256606No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23370.2573037604663837No Hit
GTATTACTGTCATTGTCAGCAGTATGGTGCCTTGCCTAAGACGTTCGGCC21650.23836655601614065No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18670.20555674830583584No Hit
ATATTACTGTGCGCGAGGCAGACCGACGTATAACGATTTAGCGGGCTACTACTACTACGGTATGGACGTCTGGGG15370.16922373976757885No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA15100.1662510390689942No Hit
GTATGGTGCCTTGCCTAAGACGTTCGGCCAAGGTACCAGGGTTGATATGAAACGAACTGTGGCTGCACCAT15000.16515003881025914No Hit
CATATTACTGTGCGCGAGGCAGACCGACGTATAACGATTTAGCGGGCTAC14250.15689253686974616No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13310.14654313443763659No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACGCAGTGCCA13060.14379063379079896No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13060.14379063379079896No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13040.14357043373905193No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12930.14235933345444335No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12740.14026743296284674No Hit
GTACCAGGGTTGATATGAAACGAACTGTGGCTGCACCATCTGTCTTCATC12670.13949673278173222No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12140.1336614314104364No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11850.13046853066010472No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11730.12914733034962264No Hit
GTAATATGTGCCCGTGTCCGCGGCGGTCACAGAGCTCAGTCCCAGGGAGA11610.12782613003914056No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11560.127275629909773No Hit
GCCTAAGACGTTCGGCCAAGGTACCAGGGTTGATATGAAACGAACTGTGG11380.12529382944404993No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11370.12518372941817643No Hit
GTCCCAGGGAGAACTGGTTCTTAGACGTGTCGACTGATATGGTGACTCGA11110.12232112874546526No Hit
CCCTGGGACTGAGCTCTGTGACCGCCGCGGACACGGGCACATATTACTGT11070.12188072864197123No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC11010.12122012848673021No Hit
GTGTATTACTGTCATTGTCAGCAGTATGGTGCCTTGCCTAAGACGTTCGGCCAAGGTACCAGGGTTGATATGAAA10850.1194585280727541No Hit
GTCTTAGGCAAGGCACCATACTGCTGACAATGACAGTAATACACTGCAAA10840.11934842804688058No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10670.11747672760703098No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10490.11549492714130788No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10420.11472422696019334No Hit
CATCTATGGTGCCTCCAGCAGGGCCACTGGCGTCCCAGCCAGGTTCAGTGGCACTGCGTCTGGGACAGACTTCA10420.11472422696019334No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10010.11021012589937959No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC9970.10976972579588556No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9970.10976972579588556No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9890.10888892558889751No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9680.10657682504555388No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAACAGTCCTGCGCCCCACTGCTGTAACTGCAC9640.10613642494205985No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9560.10525562473507181No Hit
CTCCTGCAGGGCCAGTCAGCGTGTTGACAGCACCTACTTGACCTGGTTCC9530.1049253246574513No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9410.10360412434696922No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9190.10118192377775209No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9170.10096172372600508No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAACGAGCCACCCTCTCCTGCAGGGCCAGTCAGCGTGTTGACAGCA9100.10019102354489054No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9100.10019102354489054No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9100.10019102354489054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4000.039.0349661
GGTATCA10300.038.227571
GTATCAA25500.037.670571
TGGTATC4100.036.403052
TGGATTG14550.036.15247370
CAGTTCG7400.031.49272770
ATCAACG30950.030.9036313
AACGCAG31450.030.526896
TCAACGC31500.030.3640464
CAACGCA31600.030.2729575
ACCGCGA450.005018355330.155348
ACGCAGA32500.029.5406347
CGCAGAG32900.028.9752488
AGAGTAC33350.028.58427811
GCAGAGT33550.028.413889
GAAACGA13300.028.0356370
GACTGAT8000.027.46615870
TTAACGG756.417421E-527.43590435
CAGAGTA35300.027.00525910
TATCAAC35950.026.985852
Produced by FastQC (version 0.11.5)