FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477872
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGC18830.39403857099809153No Hit
CCTATGAGGTGACTCAGCCGCTCTCAGTGTCAGTGGCCCTGGGACAGACG18730.3919459604245488No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGGTGACTCAGCCGCTCTCAGTGT18420.38545886764656645No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCAC18160.38001808015535543No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG17560.3674624167140992No Hit
GGATTACCTGTGGGGGCAATGACATTGGAGATAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC16180.3385843907992098No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16080.3364917802256671No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14910.3120082365152175No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCACAGAGGCAGTG14000.29296548029597885No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12940.27078380821642617No Hit
CCCCTGTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCT12840.26869119764288346No Hit
CCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC12660.2649244986105066No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGGTGACT12390.2592744500619413No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11960.25027622459570764No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11590.2425335654735996No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTAGTGATGGTCAGGGTGG11400.23855760538386847No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11280.23604647269561724No Hit
GACTATTACTGTCAAATATGGTACGTCAACACTGCCTCTGTGCTTTTCGG11250.23541868952355444No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTATCT10990.2299779020323434No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTC10840.22683898617202933No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10550.22077041550875548No Hit
TTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCTGACCGATTCTCTGGCTCCAACTCGGGGAATATGGCCACC10540.2205611544514012No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10020.2096795794689791No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9950.20821475206749926No Hit
GTGTTGACGTACCATATTTGACAGTAATAGTCAGCCTCATCCCCGGCTTG9580.20047209294539126No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9370.19607761074095154No Hit
CTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9160.19168312853651187No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9010.1885442126761978No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8360.1749422439481702No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8190.17138480597314762No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGG8140.17033850068637627No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7780.16280510262162254No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7770.16259584156426826No Hit
GTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGGGATCCCTGACCG7740.16196805839220543No Hit
TCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAATATGG7630.15966618676130848No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGGTGACTCAGCCGCTCTCAGTGT7510.15715505407305722No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT7500.15694579301570297No Hit
GGCCAGGCCCCTGTGCTGGTCATTCATAGGGACAGCAGCCGGCCCTCTGG7460.15610874878628586No Hit
GTATCAACGCAGAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCT7450.1558994877289316No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7310.15296983292597183No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTGGGGGCAATGACATTGGAGAT7290.15255131081126327No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT7290.15255131081126327No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAAGCACAGAGGCAGTGT7220.15108648340978337No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7180.1502494391803663No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7160.14983091706565776No Hit
GTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGC7080.14815682860682358No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7030.14711052332005223No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG6840.1431345632303211No Hit
GACCATCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAA6540.13685673150969296No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGG6470.13539190410821308No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6450.13497338199350453No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6350.13288077141996182No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGG6280.13141594401848194No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGCAATGACATTGGAGATAAAAATGTG6150.12869555027287644No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG6120.1280677671008136No Hit
GAATATGGCCACCCTGACCATCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAATATGGTACGT5860.12262697960960257No Hit
CAGTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGCAATGACATTGGAGATAAAAATGTGCA5860.12262697960960257No Hit
TGGCCACCCTGACCATCACTAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAAATATGGTACGTCAACA5850.1224177185522483No Hit
GTCTCTGGTGGCTCCATCAGTCCTTACTACTGGAGCTGGATCCGGCAGCC5770.12074363009341414No Hit
GCCTGGTCCCTCCGCCGAAAAGCACAGAGGCAGTGTTGACGTACCATATTTGACAGTAATAGTCAGCCTCATCCC5740.12011584692135133No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC5720.11969732480664279No Hit
CCCTATGAATGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG5700.11927880269193424No Hit
GTCAAATATGGTACGTCAACACTGCCTCTGTGCTTTTCGGCGGAGGGACC5660.11844175846251716No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA5580.116767670003683No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5520.11551210365955736No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG5500.11509358154484883No Hit
CGTCAACACTGCCTCTGTGCTTTTCGGCGGAGGGACCAGGCTGACCGTCC5490.11488432048749457No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG5480.1146750594301403No Hit
GTGTCAGTGGCCCTGGGACAGACGGCCAGGATTACCTGTGGGGGCAATGA5450.11404727625807748No Hit
CTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5440.11383801520072322No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5440.11383801520072322No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC5370.11237318779924331No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG5220.10923427193892926No Hit
CCTCATAGGAGGCCACAGAACCTGTAAAGTGAGCAAGGAGGCTCAGAAGGAGAGCGGTCCAGGCCATGGCTGCGG5180.10839722770951217No Hit
CAGGTAATCCTGGCCGTCTGTCCCAGGGCCACTGACACTGAGAGCGGCTGAGTCACCTCATAGGAGGCCACAGAA5180.10839722770951217No Hit
GGGTGGCCATATTCCCCGAGTTGGAGCCAGAGAATCGGTCAGGGATCCCA5040.10546757290655238No Hit
GACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTT4990.10442126761978103No Hit
CTATTACTGTCAAATATGGTACGTCAACACTGCCTCTGTGCTTTTCGGCG4900.1025379181035926No Hit
GGAAGAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC4900.1025379181035926No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA4800.10044530753004989No Hit
CTGGGATCCCTGACCGATTCTCTGGCTCCAACTCGGGGAATATGGCCACC4800.10044530753004989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAA150.002267264369.66135447
AGGGGGG2050.063.42650670
CTCTTAT850.056.1732561
CGTTCAC200.00766707651.21138827
TGTTAGA400.00930276848.7591370
TCTTATA1250.046.36852
GGTATCA3800.045.772761
GTATCAA11450.041.7006721
TCGTATG2200.039.19486640
CACGAGA2200.038.74373221
AGTGTTA400.001502471738.56106668
GAGTTTA1700.038.1175651
CGGGTAC451.07283406E-437.882764
ACGAGAC2350.037.72155822
ATCTCGT2200.037.50004237
CGAGACC2300.037.05922323
TATACAC2300.037.0592233
ACACATC2400.036.9395566
ATGCCGT2350.036.88437344
GTATGCC2350.036.76310742
Produced by FastQC (version 0.11.5)