FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214613
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35690.29383844895452293No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30110.2478978901098539No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29930.24641593659873556No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24860.20467424603556852No Hit
ACCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCC24220.1994050779960366No Hit
GAAATACACAGCCGAGTCCGCGGCGTTCACAGAGCTCAACTTCAGGGAGA23320.19199531044044482No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23220.19117200293426795No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21420.1763524678230844No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG21020.17305923779837692No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20850.17165961503787625No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20610.1696836770230518No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20050.16507315498846134No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19690.16210924796622464No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGCTCTGGGACAGACTTCA19520.16070962520572396No Hit
GTGTATTTCTGTGCGAGAGGCCCGTTTTTATATGGTTCGGGGAGACCTCCGTTTGACTCCTGGGGCCAGGGAACC18960.1560991031711335No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC18890.15552278791680973No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18720.15412316515630906No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18510.1523942193933376No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18510.1523942193933376No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17980.14803068961060026No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17790.1464664053488642No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17690.14564309784268734No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC17450.14366715982786288No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17170.14136189881056765No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT17140.1411149065587146No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC16980.1397976145488316No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16890.1390566377932724No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA16450.13543408476609423No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16430.13526942326485886No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16410.13510476176362346No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16070.13230551624262213No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16060.13222318549200446No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15720.1294239399710031No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15680.12909461696853236No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15610.12851830171420855No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15430.1270363482030902No Hit
CTACTGGACCTGGATCCGCCAGTCCCCAGGGAAGGGGCCGGAGTGGATTGGGGAAATCAGTCACAGTGGAATCAC15430.1270363482030902No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15360.1264600329487664No Hit
GGGTTGTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC15350.12637770219814873No Hit
GCACAACCCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT15090.12423710268208886No Hit
GTTTTACTGTCAGCAATATGGTAGTTCACCCTACACTTTTGGCCAGGGGA15090.12423710268208886No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14970.12324913367467662No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14860.12234349541788209No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14730.12127319565985215No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14510.11946191914626306No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14460.11905026539317462No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14340.11806229638576238No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT14340.11806229638576238No Hit
GGTCTACTGATATGGTGACTCGACTCTTGAGTGACGGGTTGTAGCTGGTG14280.11756831188205626No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14270.11748598113143857No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC14230.11715665812896782No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACTTTGCTGTGTTTTACTGTCAGCAATATGGTAGTTCA14220.11707432737835015No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14050.11567470461784947No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14030.1155100431166141No Hit
GTGTCTGGTGGGTCCTTCAGTGGTTACTACTGGACCTGGATCCGCCAGTC13800.11361643585240731No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGGTTGTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGA13770.11336944360055425No Hit
CAGTAGACCCGTCCAAGGACCAGTTCTCCCTGAAGTTGAGCTCTGTGAACGCCGCGGACTCGGCTGTGTATTT13740.11312245134870119No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13570.11172282858820051No Hit
ATATGGTAGTTCACCCTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT13060.10752396030669852No Hit
GTGTAGGGTGAACTACCATATTGCTGACAGTAAAACACAGCAAAGTCTTC12950.10661832204990397No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12860.10587734529434478No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCACAACCCTGGC12720.10472471478569717No Hit
GGTTACTACTGGACCTGGATCCGCCAGTCCCCAGGGAAGGGGCCGGAGTG12660.10423073028199105No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12580.10357208427704957No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGGAGTCAAACGGAGGTCTCC12550.1033250920251965No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12510.10299576902272575No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12390.10200780001531351No Hit
GAGTGAAGTCTGTCCCAGAGCCACTGCCACTGAACCTGTCTGGGATGCCA12350.10167847701284277No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12350.10167847701284277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA150.002380384468.81792437
GGTATCA9900.046.6102181
TCGTATG3100.044.54825240
CGTATGC3200.044.2939841
TATACAC3300.042.4531023
TATCTCG3100.041.01605236
GACGGAC3500.040.05359626
ATCTCGT3300.038.5797537
GTATCAA25550.038.261121
ACGAGAC3800.037.76947422
ATGCCGT3600.037.5754644
GTATGCC3850.036.84339542
CGAGACG3900.036.8025423
CCCACGA3750.036.4505419
GCCGTCT3850.036.12093446
CGAGCCC3800.035.96796415
CACGAGA3900.035.92481221
ATCTCCG4000.035.87656810
ACTCCTA3950.035.55937231
CTTATAC3850.035.5125471
Produced by FastQC (version 0.11.5)