FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352557
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11720.33242851510535887No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC9190.26066706943841705No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9110.2583979328165375No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8660.2456340393184648No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8520.24166305023017556No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT8230.23343742997586203No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG8060.22861551465436794No Hit
GCCTTGGGCTGACCTAGGACGATCAACTTGGTCCCTCCGCCGAATACCGAACGACGCAGACTGAGGTCATAGGA8020.22748094634342814No Hit
CTCCTGCACTGGGGACAGCTCCAATATCGGGGCTGGTTTTGATGTCCACTGGTACCAGCACTTTCGAGGAAGAGC7820.2218081047887292No Hit
GACCTAGGACGATCAACTTGGTCCCTCCGCCGAATACCGAACGACGCAGA7690.21812075777817488No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7570.2147170528453555No Hit
GAGTATTACTGCCAGTCCTATGACCTCAGTCTGCGTCGTTCGGTATTCGG7570.2147170528453555No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7490.21244791622347595No Hit
GTATTACTGCCAGTCCTATGACCTCAGTCTGCGTCGTTCGGTATTCGGCG7300.2070587167465119No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7290.20677507466877695No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAACTTGGTCCCTC6880.1951457494816441No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6840.19401118117070432No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG6670.1891892658492102No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6410.18181457182810157No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5730.16252691054212512No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC5680.16110870015345036No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC5640.15997413184251058No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT5610.15912320560930573No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG5610.15912320560930573No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT5390.15288307989913688No Hit
GTATTCGGCGGAGGGACCAAGTTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC5350.1517485115881971No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA5310.15061394327725727No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA5170.14664295418896803No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG5100.14465745964482338No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG5040.1429556071784137No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT4870.1381336918569196No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT4740.13444634484636528No Hit
GGATGAGGCTGAGTATTACTGCCAGTCCTATGACCTCAGTCTGCGTCGTT4700.13331177653542547No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG4650.13189356614675074No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4640.13160992406901578No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT4510.12792257705846147No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG4350.12338430381470229No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG4320.12253337758149746No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC4240.12026424095961787No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGAGTATTACTGCCAGT4190.11884603057094315No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT4050.11487504148265386No Hit
GGGCAGGGGGTCACCATCTCCTGCACTGGGGACAGCTCCAATATCGGGGCTGGTTTTGATGTCCACTGGTACCAG4020.11402411524944903No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT4000.11345683109397914No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG3990.11317318901624418No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG3970.11260590486077428No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA3960.11232226278303935No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG3840.10891855785021996No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC3830.10863491577248502No Hit
CATCTATGCTGATAACACCCGGCCCTCGGGGGTCCCCGACCGGATCTCTGCCTCCAAGTCTGGCACCTCAGCCTC3800.10778398953928017No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC3800.10778398953928017No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA3780.10721670538381027No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA3730.10579849499513554No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG3680.1043802846064608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.10324571629552101No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC3600.10211114798458121No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC3590.10182750590684628No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC3570.10126022175137638No Hit
CAGTAATACTCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGA3530.10012565344043658No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT100.0099736129.3325270
TCTCGTA1000.051.48782738
CGTCTTC1100.050.9371248
ACGCGTA1000.050.91021727
TAGTACG200.00788597250.8452344
AGGGGGG1700.049.4506770
CGTAGTA1100.049.4376930
ATCTCGT1050.048.96570237
GGGCGTT400.00949700648.49969570
AGACGCG1050.048.45151525
ATCTCCG1150.047.16079710
CGTATGC1100.047.0571741
CTTATAC1300.046.947381
TCGTATG1100.046.9419540
GGTATCA5100.046.538251
CGCGTAG1100.046.30174328
GACGCGT1100.046.25574526
CGAGACG1100.046.22949623
GTATTAT306.8683526E-445.2085881
AATCTCG1150.044.6437936
Produced by FastQC (version 0.11.5)