FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012722
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59160.5841682120068489No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54270.5358825027993862No Hit
GTATAATAACTGGTTTGTGATCAGCTTCGGCCAAGGGACACGACTGGAGATCAAACGAACTGTGGCTGCACCAT47420.46824301239629434No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA46980.46389828600543875No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCCTCGGACTGCAGGCTGCTGATGGTGAGAGTGAAGT40450.39941859661387824No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39680.39181532542988107No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37800.3732514944871347No Hit
CATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA35940.354885151107609No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33010.32595322309577557No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32890.32476829771645127No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31400.3100554742565087No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCCGAGGATT31190.30798185484269125No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCAGTGGCCCTGGT30980.3059082354288739No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30890.3050195413943807No Hit
CCATAGATGAGGAGCCTGGGGGACCGGCCAGGTTTCTGTTGGTACCAGGC30040.2966263199575007No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29490.2911954119689313No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29420.2905042054976588No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29020.28655445423324466No Hit
GTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAAGGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAG28670.2830984218768823No Hit
CTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCT28040.2768775636354301No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27760.2741127377503402No Hit
CTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT27480.27134791186525026No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC25170.2485380983132587No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24480.24172477738214435No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24400.24093482712926156No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24380.24073733956604085No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGTTTGTGATCAGCTTCGGCCA24360.2405398520028201No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24060.2375775385545095No Hit
GTAATAAACTGCAAAATCCTCGGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA23740.2344177375429782No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCA22760.2247408469451636No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTCGTGTCCCTTGGCCGAAGCTGATCACAAACCAGTTATTATACTG22160.21881622004854243No Hit
GTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTGCATCCA21800.21526144391056973No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21590.2131878244967523No Hit
GGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGT21340.21071922995649348No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20890.20627575978402762No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCCGAGGATTTTGCAGTTTA20670.20410339658859983No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT20040.19788253834714759No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19810.19561143137010947No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19100.1886006228757744No Hit
GTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTG18640.18405840892169817No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18510.18277473976076355No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18260.18030614522050473No Hit
GGATATCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGGGACCGGCCAGGTTTCTGTTGGTACCAGG18180.17951619496762192No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTCGTGTCC17980.17754131933541484No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17940.17714634420897343No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGGTGACGCAGTC17170.16954307302497626No Hit
CAGTAATAAACTGCAAAATCCTCGGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16540.16332221478352402No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16500.1629272396570826No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16120.1591749759558892No Hit
GGGGAAAGGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAAC15910.15710135654207177No Hit
TCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT15870.15670638141563034No Hit
GGACACGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC15690.15492899334664398No Hit
CCCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGATATCCCA15110.1492018540132435No Hit
CTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCT14980.14791818485230893No Hit
GTCTGCGTCTCCAGGGGAAAGGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTA14970.14781944107069858No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14930.14742446594425715No Hit
GTCGTGTCCCTTGGCCGAAGCTGATCACAAACCAGTTATTATACTGCTGA14850.1466345156913743No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14790.14604205300171222No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14780.14594330922010187No Hit
GGAGAAATAGTGGTGACGCAGTCTCCAGCCACCCTGTCTGCGTCTCCAGG14660.14475838384077763No Hit
ACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTT14540.1435734584614534No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14480.14298099577179127No Hit
GAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTGCATC14040.13863626938093573No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14030.1385375255993254No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13990.13814255047288396No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13790.1361676748406769No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13750.1357726997142355No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGGTGACGCAGTCTCCAGCCACC13720.13547646836940444No Hit
CACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG13670.13498274946135266No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13650.13478526189813198No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGGTGACGCAGTCTC13530.13360033651880773No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13500.13330410517397667No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13500.13330410517397667No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13440.13271164248431455No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13400.13231666735787315No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT13360.13192169223143171No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13310.13142797332337997No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13280.1311317419785489No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT13220.13053927928888678No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13100.12935435390956254No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12830.12668827180608302No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12780.12619455289803125No Hit
CGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT12730.12570083398997947No Hit
CCCAGATACCACTGGAGAAATAGTGGTGACGCAGTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAAGGGTCA12620.1246146523922656No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12580.12421967726582417No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12550.12392344592099312No Hit
TTATTACTGTCAGCAGTATAATAACTGGTTTGTGATCAGCTTCGGCCAAGGGACACGACTGGAGATCAAACGAA12470.1231334956681103No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12290.12135610759912395No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12260.12105987625429288No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC12210.12056615734624113No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12210.12056615734624113No Hit
GCTTCGGCCAAGGGACACGACTGGAGATCAAACGAACTGTGGCTGCACCA12120.11967746331174794No Hit
CTGCAGGAGAGAGTGACCCTTTCCCCTGGAGACGCAGACAGGGTGGCTGG12030.11878876927725475No Hit
GTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGC11910.11760384389793054No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11830.11681389364504771No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11590.11444404288639923No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11590.11444404288639923No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11480.11335786128868533No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11230.11088926674842652No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11130.10990182893232299No Hit
GCCTGGGGGACCGGCCAGGTTTCTGTTGGTACCAGGCCAAGTTGCTCCTA11070.10930936624266087No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10850.10713700304723309No Hit
GCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGGGACCGGCCAGGTTTCTGTTGGTACCAGGCCAAGTTGCTC10800.10664328413918134No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGGTGACG10740.10605082144951922No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10680.1054583587598571No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10660.1052608711966364No Hit
CCCTGTCTGCGTCTCCAGGGGAAAGGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGC10510.10377971447248109No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10350.10219981396671544No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGGTGACGCAGTCTCCAGCCACCCTGT10300.10170609505866367No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10270.10140986371383262No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG10150.10022493833450838No Hit
GGTTTCTGTTGGTACCAGGCCAAGTTGCTCCTAACACTCTGACTGGTCCTGCAGGAGAGAGTGACCCTTTCCC10140.10012619455289802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)