FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012722
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61260.604904406145023No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52940.5227495798452092No Hit
GTATAATAACTGGTTTGTGATCAGCTTCGGCCAAGGGACACGACTGGAGATCAAACGAACTGTGGCTGCACCAT45670.45096285061448255No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA44450.4389161092580195No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38950.38460702937232527No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCCTCGGACTGCAGGCTGCTGATGGTGAGAGTGAAGT37140.36673440490085135No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35770.353206506820233No Hit
CATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA32900.32486704149806167No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32460.32052231510720613No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31220.30827808618752234No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30870.30482205383115996No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29570.2919853622218141No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGTCCGAGGATT29120.2875418920493482No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCAGTGGCCCTGGT28300.27944490195729926No Hit
CTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT27580.2723353496813538No Hit
CTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCT27330.269866755141095No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26990.266509466566343No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26900.26562077253184985No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26410.2607823272329425No Hit
CCATAGATGAGGAGCCTGGGGGACCGGCCAGGTTTCTGTTGGTACCAGGC26220.25890619538234577No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC25100.24784689184198624No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24670.24360090923274108No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24650.24340342166952036No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24580.2427122151982479No Hit
GTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAAGGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAG24480.24172477738214435No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24380.24073733956604085No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23850.23550391914069213No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTCGTGTCCCTTGGCCGAAGCTGATCACAAACCAGTTATTATACTG23210.22918431711762954No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATATCA22820.22533330963482576No Hit
GTAATAAACTGCAAAATCCTCGGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22770.22483959072677398No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22600.22316094643939796No Hit
GTTTATTACTGTCAGCAGTATAATAACTGGTTTGTGATCAGCTTCGGCCA21730.21457023743929726No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21670.21397777474963514No Hit
GTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTGCATCCA20760.20499209062309298No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGTCCGAGGATTTTGCAGTTTA20530.20272098364605487No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20250.199956157760965No Hit
GGAGAAATAGTGGTGACGCAGTCTCCAGCCACCCTGTCTGCGTCTCCAGG20230.19975867019774432No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT19720.1947227373356163No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18820.1858357969906845No Hit
GGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGT18550.183169714887205No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18140.17912121984118048No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18080.1785287571515184No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18020.17793629446185627No Hit
GGATATCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGGGACCGGCCAGGTTTCTGTTGGTACCAGG17670.1744802621054939No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17550.17329533672616967No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17500.1728016178181179No Hit
GTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTG17360.17141920487557294No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16880.166679503358276No Hit
GGACACGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC16820.1660870406686139No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGGTGACGCAGTC16750.16539583419734144No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTCGTGTCC16100.15897748839266845No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16000.15799005057656493No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15660.15463276200181295No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15610.15413904309376117No Hit
GGGGAAAGGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAAC15560.15364532418570942No Hit
GTCTGCGTCTCCAGGGGAAAGGGTCACTCTCTCCTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTA15050.1486093913235814No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14950.14762195350747787No Hit
GTCGTGTCCCTTGGCCGAAGCTGATCACAAACCAGTTATTATACTGCTGA14910.14722697838103646No Hit
CAGTAATAAACTGCAAAATCCTCGGACTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14740.14554833409366044No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGGTGACGCAGTCTCCAGCCACC14120.13942621963381857No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14090.1391299882889875No Hit
CTGCAGGACCAGTCAGAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCT13980.1380438066912736No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13820.13646390618550797No Hit
ACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTT13790.1361676748406769No Hit
CACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG13710.1353777245877941No Hit
TCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT13700.13527898080618372No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13660.13488400567974232No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13590.13419279920846983No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13500.13330410517397667No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13290.13123048576015928No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13280.1311317419785489No Hit
CCCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGATATCCCA13080.12915686634634185No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13050.12886063500151076No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13030.12866314743829008No Hit
GAGTGTTAGGAGCAACTTGGCCTGGTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTGCATC12960.12797194096701758No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTT12720.12560209020836913No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGGTGACGCAGTCTC12390.12234354541522748No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12390.12234354541522748No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC12380.12224480163361712No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12350.12194857028878607No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12260.12105987625429288No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12250.12096113247268253No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12090.11938123196691688No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12080.11928248818530653No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12070.11918374440369618No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11980.118295050369203No Hit
CGACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT11920.11770258767954088No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11780.11632017473699593No Hit
GCTTCGGCCAAGGGACACGACTGGAGATCAAACGAACTGTGGCTGCACCA11760.11612268717377523No Hit
CCCAGATACCACTGGAGAAATAGTGGTGACGCAGTCTCCAGCCACCCTGTCTGCGTCTCCAGGGGAAAGGGTCA11710.11562896826572346No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT11700.11553022448411311No Hit
GGCCGAAGCTGATCACAAACCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATCCTCGGACTGCAGGCTG11660.1151352493576717No Hit
TTATTACTGTCAGCAGTATAATAACTGGTTTGTGATCAGCTTCGGCCAAGGGACACGACTGGAGATCAAACGAA11470.11325911750707499No Hit
GCCTGGGGGACCGGCCAGGTTTCTGTTGGTACCAGGCCAAGTTGCTCCTA11440.11296288616224394No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11410.11266665481741288No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11270.11128424187486793No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11250.11108675431164723No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11160.11019806027715404No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11130.10990182893232299No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC10970.10832192842655734No Hit
ATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGGTGACG10950.10812444086333664No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10600.10466840850697427No Hit
CACCATCAGCAGCCTGCAGTCCGAGGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGGTTTGTGATCAG10540.10407594581731214No Hit
GCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGGGACCGGCCAGGTTTCTGTTGGTACCAGGCCAAGTTGCTC10520.10387845825409145No Hit
CTCCAGTCGTGTCCCTTGGCCGAAGCTGATCACAAACCAGTTATTATACTGCTGACAGTAATAAACTGCAAAATC10490.10358222690926039No Hit
GTACCAACAGAAACCTGGCCGGTCCCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGATATCCCAGC10420.10289102043798792No Hit
GGTTTCTGTTGGTACCAGGCCAAGTTGCTCCTAACACTCTGACTGGTCCTGCAGGAGAGAGTGACCCTTTCCC10420.10289102043798792No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10320.1019035826218844No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10270.10140986371383262No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGGTGACGCAGTCTCCAGCCACCCTGT10260.10131111993222228No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10230.10101488858739122No Hit
CTGCAGGAGAGAGTGACCCTTTCCCCTGGAGACGCAGACAGGGTGGCTGG10160.10032368211611875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG200.00277953266.1766969
AATGGTA4300.043.33849770
GGTATCA10550.040.8327981
GTATCAA27800.038.556731
TGCGTAG500.00232162935.2942369
TATCAAC30950.034.3810582
TCAACGC31400.033.4549874
ATCAACG31600.033.3504833
CAACGCA31600.033.144195
AACGCAG31750.032.9908646
CGCAGAG32500.032.2295388
AGAGTAC33100.031.9525511
GCGAAAA500.004064207431.4835168
ATTAGAG1400.031.4661883
GCAGAGT33550.031.422949
TGTTAGA15950.030.72388870
GAGTAGA900.00461346430.67580670
ACGTAAA450.005028021530.14380516
GTATTAG2200.029.2946831
AAAGTCG500.00688080228.26918654
Produced by FastQC (version 0.11.5)