FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899631
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCGGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA71750.7975492174013568No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG67070.7455278886565714No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37110.4125024593416634No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC34200.38015586390420075No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33430.3715967991320886No Hit
GTGCAGGGAGAAGGGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30100.3345816229098375No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTC29310.32580024476702113No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28950.32179860409434535No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27580.3065701382011069No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC26710.2968995065754737No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT25390.2822268241089958No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24910.27689130321209476No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24410.2713334689444895No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23680.2632190309137858No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC23040.256105003051251No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGG22690.25221451906392733No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22100.24565627462815312No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21520.239209186877731No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21060.23409597935153412No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20700.2300943386788583No Hit
GAGAAGGGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20640.22942739856674568No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20560.22853814508392886No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19880.22097949047998566No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT19640.21831173003153517No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19300.21453240272956356No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18690.20775184492308513No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCGC18690.20775184492308513No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18610.2068625914402683No Hit
ATACCACACCATTCAGGCTGTCATCCCATGCCGCACAGTAATAATCAGCC18490.20552871121604302No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18450.2050840844746346No Hit
GGCTTAATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA18440.20497292778928247No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC18370.20419483099181776No Hit
GTATTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGCGACCCT17990.19997087694843774No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17630.19596923627576193No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17430.19374610256871982No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT17010.18907752178393142No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17000.1889663650985793No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16900.18785479824505824No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16680.18540935116731191No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT16520.18363084420167824No Hit
CCCCTGAGGGCCGCTGATTATTAGTATAAATGAGGAGTTTGGGGGCCGTTCCTGAGAGATGTTGGTACCAGTTTA16480.18318621746026983No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCGGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGAC16260.1807407703825235No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA15770.17529409280027033No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG15770.17529409280027033No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT15420.17140360881294664No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15420.17140360881294664No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14990.1666238713428061No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC14430.16039909696308818No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13940.15495241938083504No Hit
GGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCC13850.15395200921266608No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC13760.15295159904449715No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG13740.15272928567379293No Hit
GAAGTAATACTGTAAACTGGTACCAACATCTCTCAGGAACGGCCCCCAAA13700.1522846589323845No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATACTGTAAACTGGTACCAACATCTCTCAGGAACGGCCCC13470.14972805516928608No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC13340.14828301825970872No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTC13140.14605988455266658No Hit
TTATTACTGTGCGGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCG12830.14261402730675132No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC12740.1416136171385824No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12700.14116899039717395No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12580.1398351101729487No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12570.13972395348759659No Hit
CCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCA12570.13972395348759659No Hit
CATTTATACTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC12540.13939048343154026No Hit
ACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGC12540.13939048343154026No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12460.1385012299487234No Hit
CCATTCAGGCTGTCATCCCATGCCGCACAGTAATAATCAGCCTCATCCTC12210.13572231281492078No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC12200.13561115612956867No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCGG12150.13505537270280815No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG12010.13349917910787867No Hit
GTTTGGGGGCCGTTCCTGAGAGATGTTGGTACCAGTTTACAGTATTACTT11950.13283223899576604No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11930.13260992562506183No Hit
CAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA11850.13172067214224498No Hit
CTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG11730.13038679191801972No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11660.12960869512055498No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT11490.1277190314695692No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11450.12727440472816076No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11440.12716324804280865No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11370.1263851512453439No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA11360.1262739945599918No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG11260.12516242770647076No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11160.12405086085294972No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC10960.12182772714590759No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCGCCATCTCTTGTTCTGG10920.12138310040449918No Hit
GTATAAATGAGGAGTTTGGGGGCCGTTCCTGAGAGATGTTGGTACCAGTTTACAGTATTACTTCCGATGTTGGAG10770.11971575012421759No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGG10670.11860418327069654No Hit
GATTATTAGTATAAATGAGGAGTTTGGGGGCCGTTCCTGAGAGATGTTGG10590.11771492978787969No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC10540.11715914636111918No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTAATGCCTTGGG10510.11682567630506284No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAAC10500.11671451961971074No Hit
GGACCAAGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG10410.1157141094515418No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA10320.11471369928337284No Hit
GCTTGGTCCCTCCGCCGAATACCACACCATTCAGGCTGTCATCCCATGCCGCACAGTAATAATCAGCCTCATCCT10200.11337981905914758No Hit
GGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC9940.11048974523999283No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC9840.10937817838647178No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9700.1078219847915423No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9610.10682157462337336No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG9550.10615463451126074No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC9470.10526538102844389No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9440.10493191097238758No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG9280.1031534040067539No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9250.10281993395069758No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT9250.10281993395069758No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGG9170.10193068046788073No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC9140.1015972104118244No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC9070.10081911361435966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT250.001461023877.82075570
AGGGGGG3100.075.310470
GGTATCA7550.043.8574451
GTATCAA20550.040.8640861
TCGTATG3500.040.4358940
CGAGACA3650.038.3280223
CCGTCTT3850.038.00050747
ACATGCG3700.037.8416227
TATACAC3700.037.807973
ATCTCCG3750.037.30386410
TTATACA3750.037.3038642
CGTATGC3800.037.30231541
CGCAGAT3800.036.9320832
ATGCGCA3800.036.8724529
CACGAGA3800.036.81506721
TCTCGTA3500.036.40699438
TGGTATC2550.036.126412
TATCAAC23350.036.092032
CTTGAAA3950.035.90012457
CCCACGA3900.035.87109419
Produced by FastQC (version 0.11.5)