FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934832
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31890.3411308128091465No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31690.3389913909665052No Hit
GTTCAGGGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA24950.26689287486949526No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23550.25191692197100657No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22270.2382246221781026No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21940.23469457613774455No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20910.22367655364814212No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19540.20902151402604957No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGACTCACCATCACTTGCCGGACAAGTCAGACCATTAACAA19150.20484964143289916No Hit
GTAATACACAGCCGTGTCCTCGGGAGTCACCGAGTTAAACTGCAGGGAGA19110.20442175706437093No Hit
GATCTATGCTGCATCCAATTTGCAGAGTGGGGTCCCAGCAAGGTTCAGGGGCAGTGGATCTGGGACAGATTTCA18950.20271021959025792No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18830.2014265664846732No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17810.19051551508720282No Hit
GTAGGAGACAGACTCACCATCACTTGCCGGACAAGTCAGACCATTAACAACTTTTTGAATTGGTATCAGCAGAAA17720.18955277525801426No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17600.18826912215242952No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16790.17960446368973249No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16660.1782138394920157No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16640.17799989730775156No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT16480.17628835983363858No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAAGTGACCCAGTC16120.1724374005168843No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15160.16216817567220634No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15010.16056360929022542No Hit
GGGCAATGGCACCCTGGTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT14860.15895904290824447No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13970.14943861570849093No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13560.1450528009310764No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13320.1424854947199069No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13100.14013213069300154No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13090.14002515960086945No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTCAAAA12960.13863453540315265No Hit
GGCCAAAAGTGTACGGGGCAATGGCACCCTGGTGACAGTAGTAAGTTGCA12940.13842059321888853No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12670.13553237373132285No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12640.13521146045492666No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12250.13103958786177622No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12180.1302907902168518No Hit
GTGTATTACTGTGCAAGGTTGGTTGGAAATGGGGTCCTTGACCTCTGGGGCCAGGGAAACCTGGTCACCGTCTC12050.12890016601913498No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCC11620.1243004090574563No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11580.12387252468892807No Hit
GTCTGCATCTGTAGGAGACAGACTCACCATCACTTGCCGGACAAGTCAGA11550.12355161141253189No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11430.12226795830694713No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11300.12087733410923032No Hit
CCATTGCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA11220.12002156537217383No Hit
GCCCCTGAACCTTGCTGGGACCCCACTCTGCAAATTGGATGCAGCATAGA10900.11659849042394783No Hit
AGTCAGACCATTAACAACTTTTTGAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG10870.11627757714755164No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCACCAGGGTGCCATTGCC10860.11617060605541958No Hit
CACCATCACTTGCCGGACAAGTCAGACCATTAACAACTTTTTGAATTGGTATCAGCAGAAACCAGGGAAAGCCC10860.11617060605541958No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10740.11488695294983485No Hit
CTCCTGATCTATGCTGCATCCAATTTGCAGAGTGGGGTCCCAGCAAGGTT10720.11467301076557071No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCCCTGAACCTTGCTGGGACCCCA10690.11435209748917453No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAAGTGACCCAGTCTCCATCCTCC10650.11392421312064627No Hit
ACTTACTACTGTCACCAGGGTGCCATTGCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA10570.11306844438358978No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10250.10964536943536378No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10100.10804080305338284No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10070.10771988977698665No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10050.10750594759272256No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10010.10707806322419429No Hit
CAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCC10010.10707806322419429No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9900.10590138121074162No Hit
ATGGCACCCTGGTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC9790.10472469919728891No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG9670.10344104609170418No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9660.10333407499957212No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9560.10226436407825149No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9490.10151556643332704No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9480.101408595341195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)