FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934832
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30090.32187601622537526No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26090.27908757937255035No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24400.26100946480223186No Hit
GTTCAGGGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA23980.25651667893268526No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23790.2544842281821761No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21580.2308436168209903No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20860.2231416981874818No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19110.20442175706437093No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18820.2013195953925411No Hit
GATCTATGCTGCATCCAATTTGCAGAGTGGGGTCCCAGCAAGGTTCAGGGGCAGTGGATCTGGGACAGATTTCA18330.19607801187807006No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGACTCACCATCACTTGCCGGACAAGTCAGACCATTAACAA17550.18773426669176924No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17540.18762729559963714No Hit
GTAATACACAGCCGTGTCCTCGGGAGTCACCGAGTTAAACTGCAGGGAGA16720.17885566604480804No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16390.17532562000445No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16200.17329316925394084No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16110.17233042942475227No Hit
GTAGGAGACAGACTCACCATCACTTGCCGGACAAGTCAGACCATTAACAACTTTTTGAATTGGTATCAGCAGAAA15520.1660191349889606No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAAGTGACCCAGTC15170.1622751467643384No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15150.16206120458007428No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14560.1557499101442826No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14450.15457322813082994No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT14240.1523268351960566No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14000.14975952898488712No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13940.14911770243209477No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13660.14612251185239702No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13560.1450528009310764No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13520.14462491656254814No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13020.139276361955945No Hit
GGGCAATGGCACCCTGGTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT12990.13895544867954884No Hit
GTGTATTACTGTGCAAGGTTGGTTGGAAATGGGGTCCTTGACCTCTGGGGCCAGGGAAACCTGGTCACCGTCTC12840.1373508822975679No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12700.13585328700771904No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTCAAAA12060.12900713711126707No Hit
GTCTGCATCTGTAGGAGACAGACTCACCATCACTTGCCGGACAAGTCAGA11930.12761651291355022No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG11820.12643983090009756No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11790.12611891762370137No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCC11480.12280281376760745No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11380.12173310284628683No Hit
GGCCAAAAGTGTACGGGGCAATGGCACCCTGGTGACAGTAGTAAGTTGCA11320.12109127629349443No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11220.12002156537217383No Hit
CTCCTGATCTATGCTGCATCCAATTTGCAGAGTGGGGTCCCAGCAAGGTT10810.11563575059475927No Hit
CACCATCACTTGCCGGACAAGTCAGACCATTAACAACTTTTTGAATTGGTATCAGCAGAAACCAGGGAAAGCCC10750.1149939240419669No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10680.11424512639704246No Hit
CCATTGCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA10680.11424512639704246No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAAGTGACCCAGTCTCCATCCTCC10670.11413815530491041No Hit
ACTTACTACTGTCACCAGGGTGCCATTGCCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA10540.1127475311071936No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10490.11221267564653328No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCCCTGAACCTTGCTGGGACCCCA10470.11199873346226916No Hit
AGTCAGACCATTAACAACTTTTTGAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG10470.11199873346226916No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCACCAGGGTGCCATTGCC10450.11178479127800503No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10260.10975234052749586No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10220.1093244561589676No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10140.1084686874219111No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9970.10665017885566604No Hit
CAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCC9840.10525955465794923No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9780.10461772810515686No Hit
ATGGCACCCTGGTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC9760.10440378592089274No Hit
GTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTG9720.1039759015523645No Hit
GCCCCTGAACCTTGCTGGGACCCCACTCTGCAAATTGGATGCAGCATAGA9440.10098071097266674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGTTG5550.043.1518570
GTATCAA26550.037.6790051
TGTACGC400.00274358534.1133585
TATCAAC30600.032.3296552
ATCAACG31450.031.3440593
TCAACGC31700.030.8816624
CAACGCA32350.030.2644025
AACGCAG32350.030.2627836
ACGCAGA34300.028.5423037
CGCAGAG34600.028.4920028
AGAGTAC35950.027.89649811
TTGTACC756.6586836E-527.2877673
AATGGTA4700.026.81889770
CTGGGTT6150.026.29917769
CAGAGTA39750.025.05803110
ACGGGTA2600.024.93432816
CGAGTTA5800.024.24300831
GAGTACG31750.023.74377612
AGTACGG32000.023.3450813
TGTTAGA9850.023.03430270
Produced by FastQC (version 0.11.5)