FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615352
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28880.4693248742183335No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22520.365969396378008No Hit
GAGTTACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT21680.3523186728896632No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17970.29202797748280657No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17070.2774022023167228No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16560.26911426305594194No Hit
GTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTTCGGCCAAGGGG16280.2645640218931603No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16000.2600137807303787No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15660.2544884878898582No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15170.2465255658549903No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTC14430.2344999284962103No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTAGTAACTATTTAAATTGGTATCAGCAGAAA14190.23059972178525462No Hit
GATCTATTCTGCTTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCA13780.22393686865403864No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13470.2188991016523876No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13170.21402384326369298No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12990.21109868823047623No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12750.20719848151952053No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12580.20443583509926028No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCCCTTGGCCGAACGTC12320.20021061116239158No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12010.19517284416074052No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11970.19452280970891458No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11840.19241019774048024No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11760.19111012883682835No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11700.19013507715908942No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTAGTAA11530.18737243073882914No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11270.1831472068019605No Hit
ACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGA11080.18005954315578726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11050.1795720173169178No Hit
GTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTA10940.17778442257439644No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10900.17713438812257049No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10430.1694964833136156No Hit
ACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAA9840.1599084751491829No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9630.15649579427709667No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9470.1538956564697929No Hit
CACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTT9390.152595587566141No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9340.15178304450135857No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCC9330.15162053588840207No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT9240.15015795837179371No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA9060.14723280333857694No Hit
GAATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT8910.14479517414422965No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8760.14235754494988234No Hit
GATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATC8720.1417075104980564No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8660.14073245882031746No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTG8550.13894486407779613No Hit
GTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA8550.13894486407779613No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGA8470.1376447951741442No Hit
GTGGGGTCCCATCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCACT8250.13406960568910153No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC8250.13406960568910153No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8240.13390709707614504No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA8040.1306569248170153No Hit
GGATAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAAT8020.13033190759110233No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7920.12870682146153747No Hit
GTTCAGTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTA7810.1269192267190161No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7730.12561915781536423No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7710.12529414058945124No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7430.12074389942666962No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7330.11911881329710475No Hit
GAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTGATGGGACCCCA7090.11521860658614906No Hit
AGTCAGACCATTAGTAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATT7050.11456857213432313No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7030.11424355490841015No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6880.11180592571406285No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6810.11066836542336744No Hit
GTAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTACAACCT6760.109855822358585No Hit
GTATTATAGTGGGAGCAAAATGTACAATCCGTCCCTCGAGAGCCGAGTCA6690.10871826206788959No Hit
GCCCCTAACCTCCTGATCTATTCTGCTTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGTAGTGGAT6650.10806822761606366No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6630.1077432103901507No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6600.10725568455128122No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC6520.10595561564762931No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6490.10546808980875985No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC6380.10368049506623851No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTACTA6350.10319296922736904No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6330.10286795200145607No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6300.10238042616258662No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6170.10026781419415229No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6160.10010530558119579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)