FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1642144
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT74130.4514220433774383No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC68630.4179292437204046No Hit
GTACACAACGGCCTTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAA66990.4079422998226709No Hit
CCTTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA48230.2937014049924976No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46800.2849932770816689No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46410.2826183331059883No Hit
GTTGTGTACCTTGCATGCAGAAGTAAACCCCAATATCCTCAGCCTCTACC45150.27494543718455877No Hit
GGGTAGAGGCTGAGGATATTGGGGTTTACTTCTGCATGCAAGGTACACAACGGCCTTACACTTTTGGCCAGGGGA44990.27397110119453594No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44670.2720224292144903No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC41650.25363183740281No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40520.24675058947327397No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38250.2329271976148255No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38150.23231823762106127No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG35350.21526735779566225No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35030.21331868581561664No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34920.21264882982247596No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34900.21252703782372312No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATCAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA33760.20558489389481072No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG32920.20046962994719097No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32910.20040873394781455No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31660.19279673402576145No Hit
GTCTCCACTCTCCCTGCCCGTCACCTTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATA30030.18287068612740418No Hit
GGCCAGGCCAATCTCCAAGGCGCCTGATTTATAAGGTTTCTAACCGGGAC29950.18238351813239276No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29690.18080022214860572No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28640.1744061422140811No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC28490.1734927022234347No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT28050.17081327825087203No Hit
GTGTAAGGCCGTTGTGTACCTTGCATGCAGAAGTAAACCCCAATATCCTC27790.169229982267085No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27460.16722041428766296No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTG27320.16636787029639302No Hit
GCCTTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGG26910.1638711343219596No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26490.16131350234814973No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCC26340.16040006235750337No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA26280.16003468636124482No Hit
GCCTCTACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCC26100.15893855837246917No Hit
CTCCCTGCCCGTCACCTTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA25780.15698988639242356No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA25710.1565636143967886No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25350.15437135841923727No Hit
GGTCTAGTCAAAGCCTCGTATACGTTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC25100.15284895843482665No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25070.1526662704366974No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24610.14986505446538184No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG24150.1470638384940663No Hit
GTGTACCTTGCATGCAGAAGTAAACCCCAATATCCTCAGCCTCTACCCTGCTGATTTTCAGTGTGAAATCAGTGC23990.1460895025040435No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23780.14481068651713858No Hit
GTCCAAAGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC23380.14237484654208157No Hit
GGCCAAAAGTGTAAGGCCGTTGTGTACCTTGCATGCAGAAGTAAACCCCA22640.13786854258822612No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT22000.13397119862813492No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA21840.1329968626381121No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21790.13269238264122998No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21790.13269238264122998No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC21310.12976937467116162No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20650.12575023871231755No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20200.12300991874037842No Hit
CCACTCTCCCTGCCCGTCACCTTTGGACAGCCGGCCTCCATCTCCTGCAG19460.11850361478652298No Hit
ATAATACACGGCCGTGTCTTCCATTTCCAGGCTGTTCATTTGCAGATACA19020.11582419081396028No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC18980.1155806068164546No Hit
ATATTGGGGTTTACTTCTGCATGCAAGGTACACAACGGCCTTACACTTTT18840.11472806282518465No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT18820.11460627082643178No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTTTCCATCAACGTAT18780.11436268682892609No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18690.11381462283453826No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18560.11302297484264473No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT18470.1124749108482569No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18300.1114396788588577No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC18250.11113519886197558No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18080.11009996687257634No Hit
CCTTATAAATCAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAACCAA18080.11009996687257634No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18070.11003907087319992No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTC18070.11003907087319992No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18020.1097345908763178No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17620.1072987509012608No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17510.10662889490812011No Hit
CGTATACGTTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAG17450.10626351891186156No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17330.10553276691934446No Hit
GTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC17290.10528918292183878No Hit
GTTAGAAACCTTATAAATCAGGCGCCTTGGAGATTGGCCTGGCCTCTGCT17250.10504559892433307No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17230.10492380692558022No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTAGAGGCT17220.1048629109262038No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17050.10382767893680456No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17050.10382767893680456No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT16820.1024270709511468No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16790.10224438295301753No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16770.10212259095426467No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16720.10181811095738254No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16710.10175721495800612No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16660.101452734961124No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16550.10078287896798331No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16460.10023481497359549No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC16460.10023481497359549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3550.047.1117481
CGCTAAT3000.046.8496732
GGTATCA12700.045.6884771
TGGTATC3600.045.4940642
CGTATGC2950.044.55973441
TCGTATG2950.044.50203740
TCGCTAA3300.042.56196631
ACTCGCT3300.042.49311429
CACTCGC3450.041.61408228
ATCTCGT3150.041.53054437
AATCGAG600.001071826841.30815570
GTATGCC3450.041.1657542
ACACTCG3500.040.02584527
CTAATCT3300.039.53348534
TATACAC3650.039.2623
ATGCCGT3450.039.2425844
GCTAATC3350.038.90648333
AATGGTA5100.038.8782670
CGAGACA3700.038.7503123
ACGAGAC3750.038.2313122
Produced by FastQC (version 0.11.5)