FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1642144
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT72950.4442363154510201No Hit
GTACACAACGGCCTTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAA69600.4238361556599178No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62790.38236598008457234No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45980.27999980513280204No Hit
GTTGTGTACCTTGCATGCAGAAGTAAACCCCAATATCCTCAGCCTCTACC45860.2792690531402849No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44840.2730576612038895No Hit
GGGTAGAGGCTGAGGATATTGGGGTTTACTTCTGCATGCAAGGTACACAACGGCCTTACACTTTTGGCCAGGGGA43990.2678815012568934No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC41560.25308377340842214No Hit
CCTTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA39900.2429750375119356No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39450.2402347175399965No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38130.23219644562230837No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37640.22921254165286356No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36090.2197736617495177No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33930.2066201258842099No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33780.20570668589356356No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32890.20028694194906171No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATCAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA32630.19870364596527465No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32200.1960851179920884No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31810.1937101740164078No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG30840.1878032620768946No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30160.1836623341192977No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29460.17939961416294795No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29370.17885155016856014No Hit
GTCTCCACTCTCCCTGCCCGTCACCTTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTATA29120.1773291501841495No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28580.17404076621782255No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28500.17355359822281116No Hit
GCCTCTACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCC27420.16697683029015725No Hit
GTGTAAGGCCGTTGTGTACCTTGCATGCAGAAGTAAACCCCAATATCCTC27280.1661242862988873No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGTG27250.16594159830075803No Hit
GGCCAGGCCAATCTCCAAGGCGCCTGATTTATAAGGTTTCTAACCGGGAC27200.1656371183038759No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26310.1602173743593741No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT26060.15869497437496347No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCC25600.15589375840364791No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC25360.15443225441861372No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA24680.1502913264610168No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24650.15010863846288755No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24560.1495605744684997No Hit
GCCTTACACTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGG24430.1487689264766062No Hit
GTGTACCTTGCATGCAGAAGTAAACCCCAATATCCTCAGCCTCTACCCTGCTGATTTTCAGTGTGAAATCAGTGC24240.14761190248845413No Hit
CTCCCTGCCCGTCACCTTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA24160.1471247344934427No Hit
GGTCTAGTCAAAGCCTCGTATACGTTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCTC24040.1463939825009256No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23720.14444531052088003No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG23230.1414614065514352No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA22290.13573718261005124No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22180.13506732661691057No Hit
GGCCAAAAGTGTAAGGCCGTTGTGTACCTTGCATGCAGAAGTAAACCCCA21710.13220521464621862No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21510.1309872946586901No Hit
GTCCAAAGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC21140.12873414268176236No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20960.12763801469298672No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC20360.12398425473040123No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19740.12020870276906288No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19310.1175901747958766No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18590.11320566284077402No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18560.11302297484264473No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT18540.11290118284389189No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18520.11277939084513905No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTTTCCATCAACGTAT18510.1127184948457626No Hit
ATATTGGGGTTTACTTCTGCATGCAAGGTACACAACGGCCTTACACTTTT18510.1127184948457626No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18190.110769822865717No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT18170.11064803086696416No Hit
CCACTCTCCCTGCCCGTCACCTTTGGACAGCCGGCCTCCATCTCCTGCAG18000.10961279887756493No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17650.10748143889939006No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17430.10614172691310872No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17430.10614172691310872No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17320.10547187091996804No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17300.1053500789212152No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17290.10528918292183878No Hit
ATAATACACGGCCGTGTCTTCCATTTCCAGGCTGTTCATTTGCAGATACA17260.10510649492370949No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17250.10504559892433307No Hit
GTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC17240.10498470292495664No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17210.10480201492682738No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTC17190.10468022292807451No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16980.1034014069411696No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16980.1034014069411696No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC16970.10334051094179315No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16940.1031578229436639No Hit
CCTTATAAATCAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAACCAA16870.10273155094802891No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16610.10114825496424187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3800.047.5380441
GGTATCA13400.044.003821
TGGTATC3950.043.9735342
GTATCAA33700.038.432861
GACAGGT3250.032.510781
TCAACGC40200.031.3439924
TATCAAC40550.031.2442722
AGGTACG553.5134898E-430.961762
CAACGCA40750.030.9247115
AACGCAG41050.030.7005756
GATTCAC2150.030.1206381
ACGCAGA42600.029.6634927
CGCAGAG43200.029.093818
GGACCGT605.845082E-428.3842076
AGAGTAC44300.028.3713911
CAGAGTA44850.028.17535810
GCAGAGT45100.027.9436519
CTGTCTT43650.025.7039770
CTAAGGG801.0222005E-425.6230428
ATTCACT2600.024.888492
Produced by FastQC (version 0.11.5)