FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843839
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC30780.3647615244140174No Hit
CAGTAATATATGGCCGTGTCCGCAGCGGTCACAGAGGTCAACTTCAGGGA25530.30254586479174345No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT25460.30171632266344645No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC24600.29152480508722634No Hit
GTAATATATGGCCGTGTCCGCAGCGGTCACAGAGGTCAACTTCAGGGAGA24550.2909322749955856No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA23660.2803852393643811No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22760.26971969771484844No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC21370.2532473611672369No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC19970.23665651860129716No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19960.23653801258296903No Hit
AGCCAGGACAGGCCCCTGTAATTGTCATCTATAGTAAAAATAATCGGCCC19480.23084972370321827No Hit
ATATTACTGTGCGCGAATGGGATATTGTAGTGGTGGTAGCTGCTACCCCA18210.2157994593755444No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC17700.2097556524408092No Hit
GACCTAGGACGGTGACCCTGGTCCCAGTTCCAAAGACATAATGATTACCA16930.20063068902954234No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGTTCCCAGATGGGTC16800.19909011079127653No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC16220.19221676172824437No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16140.19126871358161923No Hit
CTATTATTGTAACTCCCGGGACAGCAGTGGTAATCATTATGTCTTTGGAA15930.18878008719672829No Hit
ATATATTACTGTGCGCGAATGGGATATTGTAGTGGTGGTAGCTGCTACCCCACCCCCTGGTTCGACCCCTGGGGC15700.18605444877518104No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15480.18344731637196196No Hit
GTCCTGGCTTCTGCTGGTACCAATTTGCATAATAACTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT15430.18285478628032123No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15430.18285478628032123No Hit
ACCTAGGACGGTGACCCTGGTCCCAGTTCCAAAGACATAATGATTACCAC14930.1769294853639142No Hit
GCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCAGTTCCAAAGACATA14700.17420384694236696No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14450.17124119648416344No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC13950.16531589556775642No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13860.16424934140280315No Hit
CTCTTTGCATAGGTTCTGTAGTTTCTTCTGAGCTGACTCAGGACCCTGCT13830.1638938233478187No Hit
TCACTAGACAGGTCCAAGAATCAGTTCTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCATATATTA13690.16223473909122477No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13630.1615237029812559No Hit
CTCCTCACTCTTTGCATAGGTTCTGTAGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA13570.16081266687128706No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13140.155716908083177No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13090.1551243779915363No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCAGTTCCAAAGACATAATGATTAC12760.15121367938670766No Hit
GTCTAGTGATATGGTGACTCGACTCTTGAGGGAGGGGTTGTAGTTGGTGCTCCCACTGTAATACATATATCCAAT12640.14979160716676998No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12460.14765849883686344No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTAGTTTCTTCTGAGCTGACTCAGGA12380.1467104506902383No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGTTATTATGCAAATTGGTA12190.14445883634200363No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12120.14362929421370665No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12000.14220722199376895No Hit
GTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCA11380.13485984885742422No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG11290.13379329469247098No Hit
CAATTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAATTTGCATAATAA11110.13166018636256444No Hit
CAATAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA11080.13130466830758No Hit
GTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACT10980.1301196081242986No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10950.12976409006931416No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10840.12846052386770462No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10680.12656442757445438No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTATTGTAACTCCCGG10670.12644592155612622No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10290.12194269285965688No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10130.12004659656640662No Hit
GGACAGGCCCCTGTAATTGTCATCTATAGTAAAAATAATCGGCCCTCAGG10060.11921705443810963No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9950.11791348823650008No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9720.11518784981495286No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9610.11388428361334331No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT9610.11388428361334331No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9590.11364727157668701No Hit
CCATATCACTAGACAGGTCCAAGAATCAGTTCTCCCTGAAGTTGACCTCT9520.11281772944839004No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9440.1118696813017649No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9410.1115141632467805No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9340.11068462111848351No Hit
CTGTAATTGTCATCTATAGTAAAAATAATCGGCCCTCAGGGATCCCAGAC9280.10997358500851465No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9250.10961806695353024No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGGGGGTGGGGTAGCAGCTACCA9230.10938105491687396No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9180.10878852482523325No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG9180.10878852482523325No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9080.10760346464195183No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9020.106892428531983No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8970.10629989844034228No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8890.10535185029371717No Hit
ATGTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT8870.10511483825706089No Hit
GTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC8650.1025077058538418No Hit
GGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTAGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGG8640.10238919983551364No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT8600.10191517576220108No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8540.10120413965223224No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8520.10096712761557594No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8520.10096712761557594No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8470.10037459752393525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)