FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843839
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC29250.34663010360981183No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC24050.28500697407917863No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT23780.2818073115843188No Hit
GTAATATATGGCCGTGTCCGCAGCGGTCACAGAGGTCAACTTCAGGGAGA22810.27031222780648917No Hit
CAGTAATATATGGCCGTGTCCGCAGCGGTCACAGAGGTCAACTTCAGGGA22650.2684161315132389No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21480.25455092736884644No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21060.24957367459906452No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC19910.2359454824913283No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18930.22433189269517054No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC18900.2239763746401861No Hit
AGCCAGGACAGGCCCCTGTAATTGTCATCTATAGTAAAAATAATCGGCCC18840.22326533853021727No Hit
ATATTACTGTGCGCGAATGGGATATTGTAGTGGTGGTAGCTGCTACCCCA17920.21236278484402832No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG17730.21011117049579364No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16910.20039367699288607No Hit
ATATATTACTGTGCGCGAATGGGATATTGTAGTGGTGGTAGCTGCTACCCCACCCCCTGGTTCGACCCCTGGGGC16510.19565343625976045No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGTTCCCAGATGGGTC16450.1949424001497916No Hit
CTATTATTGTAACTCCCGGGACAGCAGTGGTAATCATTATGTCTTTGGAA16340.19363883394818204No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15620.18510640062855593No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15200.18012914785877399No Hit
CTCCTCACTCTTTGCATAGGTTCTGTAGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA15190.18001064184044588No Hit
GACCTAGGACGGTGACCCTGGTCCCAGTTCCAAAGACATAATGATTACCA15170.17977362980378958No Hit
TCACTAGACAGGTCCAAGAATCAGTTCTCCCTGAAGTTGACCTCTGTGACCGCTGCGGACACGGCCATATATTA14850.17598143721728907No Hit
GCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCAGTTCCAAAGACATA14450.17124119648416344No Hit
GTCCTGGCTTCTGCTGGTACCAATTTGCATAATAACTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT14130.16744900389766296No Hit
CTCTTTGCATAGGTTCTGTAGTTTCTTCTGAGCTGACTCAGGACCCTGCT13880.16448635343945942No Hit
ACCTAGGACGGTGACCCTGGTCCCAGTTCCAAAGACATAATGATTACCAC13650.16176071501791217No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13280.15737599233977098No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC13160.1559539201198333No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG12880.15263575160664533No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12570.14896206503847298No Hit
GTCTAGTGATATGGTGACTCGACTCTTGAGGGAGGGGTTGTAGTTGGTGCTCCCACTGTAATACATATATCCAAT12450.14753999281853528No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12240.14505136643364433No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12120.14362929421370665No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12110.14351078819537852No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12060.1429182581037378No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCCTGGTCCCAGTTCCAAAGACATAATGATTAC11740.1391260655172373No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11640.1379410053339559No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11350.13450433080243981No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGTTATTATGCAAATTGGTA11290.13379329469247098No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11100.13154168034423627No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11080.13130466830758No Hit
GTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACT10970.13000110210597046No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTAGTTTCTTCTGAGCTGACTCAGGA10780.12774948775773576No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10470.12407580118956342No Hit
CAATAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA10450.12383878915290714No Hit
GTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCA10440.12372028313457899No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10430.12360177711625085No Hit
TCACTGGGGCTCAGGCGGAAGATGAGGCTGACTATTATTGTAACTCCCGG10060.11921705443810963No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10000.1185060183281408No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9980.11826900629148451No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9910.11743946416318753No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT9880.1170839461082031No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA9790.11601739194324985No Hit
GTATCAACGCAGAGTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATGGCCTGGACCCCT9540.11305474148504632No Hit
CAATTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAATTTGCATAATAA9470.11222519935674934No Hit
ATGTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT9180.10878852482523325No Hit
CTGTAATTGTCATCTATAGTAAAAATAATCGGCCCTCAGGGATCCCAGAC8990.10653691047699856No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8810.10440380214709205No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG8750.10369276603712319No Hit
GTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC8660.10262621187216994No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCAGGGGGTGGGGTAGCAGCTACCA8650.1025077058538418No Hit
GGACAGGCCCCTGTAATTGTCATCTATAGTAAAAATAATCGGCCCTCAGG8580.1016781637255448No Hit
GTCTCTGGTGACTCCATCAGTGATTACTACTGGAGTTGGATCCGGCAGTC8540.10120413965223224No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8490.10061160956059154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)