FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093776
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGC44500.40684747151153433No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTGCTGCTGTCCCACAC33070.3023470984918301No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG29040.2655022600605609No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26590.24310279252790332No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCGCTCTCAGTGT26590.24310279252790332No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26100.23862289902137185No Hit
GTAATACACGGCCGTGTCCGTGGCGGTCACAGAGCTCAGCGTCAGGGAGAACTGGTTCTTGGGCGTGTCTGCTGA26050.23816576703090944No Hit
CCTATGAGCTGACTCAGCCGCTCTCAGTGTCAGTGACCCTGGGACAGACG25740.23533154869004258No Hit
CAGTAATACACGGCCGTGTCCGTGGCGGTCACAGAGCTCAGCGTCAGGGAGAACTGGTTCTTGGGCGTGTCTG25010.22865742162929154No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24920.22783458404645923No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGCATT24510.22408610172466756No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGG24480.22381182253039011No Hit
GCCTATGTGGTCTTCGGCGGAGGGACTAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCT22630.2068979388832814No Hit
GTCTCTGGTGGCTCCATCAATACTTACTACTGGAGCTGGATCCGGCAGCC21870.19994953262825296No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21390.19556106551981392No Hit
GGATTACCTGTGGGGGAAACAACATTGGCATTTACAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC20860.1907154664209125No Hit
CCCCTGTGCTGGTCATCTATAACGATAACAGTCGGCCCTCTGGGATCCCT20080.1835842073696991No Hit
CCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTAGTCCCTC19990.1827613697868668No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTC19910.18202995860212695No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19890.181847105805942No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19520.17846432907652024No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18540.16950454206345725No Hit
GTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGCGG17770.1624647094103363No Hit
GTATTACTGTGCGCGAGCGTCCTTCGGTGGCGTCGATGCTTTTGATATATGGGGCCAAGGGACAATGGTCACCGT17310.15825909509808223No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17130.15661341993241762No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16870.15423633358201314No Hit
GTGTATTACTGTGCGCGAGCGTCCTTCGGTGGCGTCGATGCTTTTGATAT16630.15204210002779361No Hit
CTATTACTGTCAGGTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGGCG16550.1513106888430538No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT16470.15057927765831394No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16470.15057927765831394No Hit
GGACTAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAG16360.14957358727929668No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16100.1471965009288922No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15970.14600795775368997No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTGTAAATG15870.14509369377276518No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15730.14381372419947047No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15650.14308231301473062No Hit
CTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15560.1422594754318983No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT15500.14171091704334343No Hit
GCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCGTCATCCC15380.14061380026623366No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15020.1373224499349044No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14830.1355853483711473No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG14390.13156258685507818No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14390.13156258685507818No Hit
GTCCCACACCTGACAGTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGCGGCCGTGTTCCC14230.1300997644855985No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGG14030.12827123652374892No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13810.12625985576571438No Hit
CCACATAGGCAGTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCGTCA13620.12452275420195724No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT13420.12269422624010767No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13380.12232852064773775No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13210.1207742718801656No Hit
GGGTATATCTATTTCAGGGGGAGCACCACCTCCAAGTCCTCCCTCAAGAGTCGAGCCACCATGTCAGCAGACACG13130.12004286069542576No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13080.11958572870496337No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13040.11922002311259344No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12980.11867146472403856No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12880.11775720074311377No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12700.11611152557744914No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG12140.11099164728427027No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATAACGATAACAGTCGGCCCTCTGG12050.11016880970143796No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11830.10815742894340341No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11730.1072431649624786No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11590.10596319538918389No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11460.10477465221398166No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11410.10431752022351926No Hit
GACCCAGGACGGTCAGCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTG11340.1036775354368719No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11330.10358610903877942No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11310.10340325624259446No Hit
ACCCAGGACGGTCAGCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTGC11280.10312897704831703No Hit
CAGTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGC11170.10212328666929975No Hit
GTGTCAGTGACCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACAA11160.10203186027120727No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11050.10102616989218999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)