FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453150
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCTGTTTGGTGACTCACCCCCGGTAAAGGTGACGTTCG74460.5124040876716099No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53550.36850978907889753No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53220.36623886040670267No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA46370.3190998864535664No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCT41650.2866187248391426No Hit
GTGTATTACTGTCAGCTGTTTGGTGACTCACCCCCGGTAAAGGTGACGTT37930.2610191652616729No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32510.22372088222138115No Hit
GTGATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAGGTTCAGGGAGA32170.22138113752881672No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30650.21092110243264633No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30170.20761793345490828No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA29460.2027319960086708No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA29260.20135567560127998No Hit
CTCCTGCAGGGCCAGTCTGACTGTTAGCAACAACTACTTAGCCTGGTATC28850.19853421876612878No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28370.19523104978839073No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28010.19275367305508723No Hit
GTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCTGA27880.1918590647902832No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27480.18910642397550148No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT27370.18834944775143653No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27300.18786773560884976No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGTTGGATCCGGCAGCC27250.18752365550700204No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27160.18690431132367616No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCTGACTGTTAGCAA26090.17954099714413516No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT25580.17603138010528852No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT25550.1758249320441799No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25440.17506795582011492No Hit
CTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGC24550.1689433300072257No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGATACCAGGCTAAGTAGTTGTTGCTAACAGTCAGA23380.16089185562398928No Hit
GTCTGACTGTTAGCAACAACTACTTAGCCTGGTATCAGCACAAACCTGGC23110.15903382307401162No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22780.15676289440181673No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22340.1537349895055569No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22020.15153287685373154No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21860.15043182052781887No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC21540.14822970787599352No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC21250.1462340432852768No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT20670.14224271410384337No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA20050.13797612084093178No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19960.1373567766576059No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19900.13694388053538864No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT19690.13549874410762824No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCTGTTTGGTGACTCA19490.13412242370023741No Hit
GGTTTGTGCTGATACCAGGCTAAGTAGTTGTTGCTAACAGTCAGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC19470.13398479165949834No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19320.1329525513539552No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19140.13171386298730345No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18870.12985583043732582No Hit
CCTTTACCGGGGGTGAGTCACCAAACAGCTGACAGTAATACACTGCAAAA18050.12421291676702337No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCTCCGGAGAAAATTTGCTGACGCAGTCTC17620.12125382789113306No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC17560.1208409317689158No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17430.11994632350411176No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17400.11973987544300314No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17310.11912053125967725No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17200.11836355503561229No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACGACCCCTCCCTCAAGAG16170.11127550493754945No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16130.1110002408560713No Hit
CTGCTACTCTGGCTCCCAGATACCTCCGGAGAAAATTTGCTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTC16100.11079379279496267No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15740.10831641606165916No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15630.10755943983759418No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15610.10742180779685512No Hit
GTGTATCACTGTGCGAGACTTGGGTATAGTGGGAGCTACCCCATCAACTG15540.1069400956542683No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC15440.10625193545057289No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15340.10556377524687746No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15240.10487561504318205No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14860.10226060626913945No Hit
AACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCC14700.10115954994322678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)