FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453150
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCTGTTTGGTGACTCACCCCCGGTAAAGGTGACGTTCG72150.49650758696624575No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53800.37023018958813614No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50530.3477273509272959No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48460.3334824347108007No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA41880.28820149330764205No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCT39310.2705157760726697No Hit
GTGTATTACTGTCAGCTGTTTGGTGACTCACCCCCGGTAAAGGTGACGTT38640.2659051027079104No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33050.2274369473213364No Hit
GTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCTGA29390.202250283866084No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28770.1979836906031724No Hit
CTCCTGCAGGGCCAGTCTGACTGTTAGCAACAACTACTTAGCCTGGTATC28420.19557512989023845No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA28360.1951622337680212No Hit
GTGATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAGGTTCAGGGAGA28320.19488696968654304No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27920.19213432887176135No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA27760.19103327254584868No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27440.18883115989402333No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT27210.18724839142552385No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25520.17561848398307126No Hit
CTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGC25360.17451742765715858No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGTTGGATCCGGCAGCC25090.17265939510718095No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24890.1712830746997901No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCTGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCTGACTGTTAGCAA24770.1704572824553556No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24700.1699755703127688No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24390.16784227368131302No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT23840.1640573925609882No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGCTGATACCAGGCTAAGTAGTTGTTGCTAACAGTCAGA22470.15462959777036092No Hit
GTCTGACTGTTAGCAACAACTACTTAGCCTGGTATCAGCACAAACCTGGC22400.15414788562777415No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22110.15215222103705742No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22030.1516016928741011No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21850.15036300450744933No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21750.1496748443037539No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21040.14478890685751644No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC20840.14341258645012558No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20510.1411416577779307No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20430.14059112961497439No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20260.13942125726869214No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC20240.13928362522795307No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA19080.1313009668650862No Hit
GCACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT18560.12772253380587No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT18460.1270343736021746No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC18410.12669029350032687No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCTGTTTGGTGACTCA18390.1265526614595878No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17700.12180435605408939No Hit
GGTTTGTGCTGATACCAGGCTAAGTAGTTGTTGCTAACAGTCAGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC17660.12152909197261123No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17490.12035921962632902No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCTCCGGAGAAAATTTGCTGACGCAGTCTC17120.11781302687265596No Hit
CCTTTACCGGGGGTGAGTCACCAAACAGCTGACAGTAATACACTGCAAAA16980.11684960258748238No Hit
CTGCTACTCTGGCTCCCAGATACCTCCGGAGAAAATTTGCTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTC16890.11623025840415649No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACGACCCCTCCCTCAAGAG16680.1147851219763961No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC16370.1126518253449403No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16200.11148195299865808No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16030.11031208065237588No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15970.10989918453015862No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15820.10886694422461549No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15800.10872931218387641No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15240.10487561504318205No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15100.10391219075800848No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14920.10267350239135671No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14860.10226060626913945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGAT11150.042.58121570
AATCAAA26800.039.86030670
GTGGTAT4000.039.77881
GTATCAA31300.036.666481
TATCAAC36100.031.767092
ATCAACG36100.031.7616233
TCAACGC36650.031.378354
CAACGCA36650.031.2946765
AACGCAG37000.031.1836746
CACGCAA450.00494212830.25107230
TTCGATA450.00499928930.18002327
ACGCAGA38300.030.0368757
CGCAGAG38900.029.4865998
AGAGTAC38800.029.30098211
TAACCGT853.6354904E-629.14158253
GCAGAGT40100.028.5198299
AAATCAA25900.028.08374869
GATTCAC2200.027.6989731
AATGGTA4600.027.23680370
TTAACGG907.3160154E-626.63567735
Produced by FastQC (version 0.11.5)