FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376241
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18090.4808088432680117No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17990.4781509723820636No Hit
ATCTATTACTGTCAACAATATGGAAGCTCACCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAGAGAA13930.3702414144125707No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11170.29688417796040306No Hit
GTCTGGGACAGACTTCACTCTCAGCATCAGCAGACTGGAGCCTGACGATT11090.29475788125164454No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC10760.2859869073280158No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10040.26685023694918947No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10020.26631866277199984No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9550.2538266696080438No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9370.24904250201333722No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9200.2445241215072254No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9170.243726760241441No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT8940.23761365720376035No Hit
GTAATAGATTGTGAAATCGTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAGTCTGTCCCAGACACACTGCCA8670.23043740581170047No Hit
CTTTTGGCCAGGGGACCAACCTGGAGATCAAGAGAACTGTGGCTGCACCA8180.2174138384705548No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7910.2102375870784949No Hit
CTCCCAGACTCCTCATCTATGCTGCCTCCATCAGGGCCACTGGCGTCCCA7660.20359290986362463No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7580.20146661315486616No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7290.19375878758561665No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7040.1871141103707464No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6820.18126679442166058No Hit
GTCCCAGACACACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGAT6590.17515369138397996No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCGTCTCCTGCCGGGCCAGTCAGA6490.17249582049803186No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCTCTTGATCTCCAGGTTGGTCC6460.1716984592322474No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCGTCTCCT6460.1716984592322474No Hit
CTATTACTGTCAACAATATGGAAGCTCACCGTACACTTTTGGCCAGGGGA6400.17010373670067855No Hit
GGCCAAAAGTGTACGGTGAGCTTCCATATTGTTGACAGTAATAGATTGTG6220.16531956910597198No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6180.16425642075159272No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6170.1639906336629979No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5940.15787753062531729No Hit
GTCTGGGAGTCTGGCCAGGTCTCTGCTGATACCAGGCTAAGTAGCGACTG5730.15229600176482627No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5650.15016970505606778No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5650.15016970505606778No Hit
GACTTCACTCTCAGCATCAGCAGACTGGAGCCTGACGATTTCACAATCTATTACTGTCAACAATATGGAAGCTCA5460.1451197503727664No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5270.14006979568946498No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5210.13847507315789614No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5200.13820928606930133No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5180.13767771189211173No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5140.13661456353773246No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5070.13475405391756878No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT5010.13315933138599992No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC4890.1299698863228622No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC4850.12890673796848295No Hit
GTCCCAGACAGGTTCAGTGGCAGTGTGTCTGGGACAGACTTCACTCTCAG4840.12864095087988817No Hit
CATCTATGCTGCCTCCATCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGTGTCTGGGACAGACTTCA4820.12810937670269854No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT4760.12651465417112967No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG4700.12491993163956082No Hit
GTGCAGCCACAGTTCTCTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTG4680.1243883574623712No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4650.12359099619658676No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC4610.12252784784220752No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA4540.12066733822204385No Hit
GAGTGAAGTCTGTCCCAGACACACTGCCACTGAACCTGTCTGGGACGCCA4480.11907261569047499No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA4440.11800946733609576No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA4350.11561738353874246No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT4350.11561738353874246No Hit
GCAGAGACCTGGCCAGACTCCCAGACTCCTCATCTATGCTGCCTCCATCAGGGCCACTGGCGTCCCAGACAGGTT4340.11535159645014764No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC4310.11455423518436322No Hit
CAGTAATAGATTGTGAAATCGTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAGTCTGTCCCAGACACACTGCCA4240.11269372556419954No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4190.1113647901212255No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4150.11030164176684626No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG4110.109238493412467No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG4080.10844113214668258No Hit
CAAGAGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT4080.10844113214668258No Hit
GCATAGATGAGGAGTCTGGGAGTCTGGCCAGGTCTCTGCTGATACCAGGCTAAGTAGCGACTGCTAACAGTCTGA3980.10578326126073447No Hit
CTGCTACTCTGCCTCCCAGTCTCAAATACCACCGGAGAAGTTGTGTTGAC3970.10551747417213966No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT3950.10498589999495005No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC3910.10392275164057081No Hit
ATATGGAAGCTCACCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAGAGAACTGTGGCTGCACCAT3900.103656964551976No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC3900.103656964551976No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT3880.10312539037478637No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT3850.10232802910900193No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGCCTCCCAGT3830.10179645493181232No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC3830.10179645493181232No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC3810.1012648807546227No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA3810.1012648807546227No Hit
GACCTGGCCAGACTCCCAGACTCCTCATCTATGCTGCCTCCATCAGGGCC3790.10073330657743308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)