FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376241
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16450.4372197607384628No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14750.3920359556773451No Hit
ATCTATTACTGTCAACAATATGGAAGCTCACCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAGAGAA12550.3335627961864868No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11570.30751566150419546No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11280.2998078359349459No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC10300.2737607012526545No Hit
GTCTGGGACAGACTTCACTCTCAGCATCAGCAGACTGGAGCCTGACGATT10190.27083704327811164No Hit
CTCCCAGACTCCTCATCTATGCTGCCTCCATCAGGGCCACTGGCGTCCCA10100.26844495948075836No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9820.2610029210001037No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9470.2517003728992853No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8600.22857689619153682No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTT8350.22193221897666657No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8300.22060328353369252No Hit
GTAATAGATTGTGAAATCGTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAGTCTGTCCCAGACACACTGCCA8040.21369281923022745No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7580.20146661315486616No Hit
CTTTTGGCCAGGGGACCAACCTGGAGATCAAGAGAACTGTGGCTGCACCA7440.1977455939145388No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7340.19508772302859073No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7190.19110091669966855No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7160.19030355543388414No Hit
CATCTATGCTGCCTCCATCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGTGTCTGGGACAGACTTCA7140.1897719812566945No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6920.18392466530760868No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6700.17807734935852287No Hit
GTCCCAGACACACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGAT6640.176482626826954No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6450.17143267214365263No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6370.16930637543489413No Hit
CTATTACTGTCAACAATATGGAAGCTCACCGTACACTTTTGGCCAGGGGA6350.1687748012577045No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6340.1685090141691097No Hit
GTCTTTGTCTCCAGGGGACAGAGCCACCGTCTCCTGCCGGGCCAGTCAGA6210.16505378201737717No Hit
GTCTGGGAGTCTGGCCAGGTCTCTGCTGATACCAGGCTAAGTAGCGACTG6140.16319327239721348No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA5960.15840910480250692No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5780.15362493720780032No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGACAGAGCCACCGTCTCCT5600.14884076961309373No Hit
GACTTCACTCTCAGCATCAGCAGACTGGAGCCTGACGATTTCACAATCTATTACTGTCAACAATATGGAAGCTCA5500.14618289872714563No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5440.1445881761955768No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5440.1445881761955768No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5430.14432238910698197No Hit
GGCCAAAAGTGTACGGTGAGCTTCCATATTGTTGACAGTAATAGATTGTG5410.14379081492979234No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCTCTTGATCTCCAGGTTGGTCC5330.14166451822103387No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5060.134488266828974No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4920.13076724758864663No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4890.1299698863228622No Hit
CTGCTACTCTGCCTCCCAGTCTCAAATACCACCGGAGAAGTTGTGTTGAC4860.12917252505707777No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4810.12784358961410372No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA4710.12518571872815562No Hit
GTCCCAGACAGGTTCAGTGGCAGTGTGTCTGGGACAGACTTCACTCTCAG4610.12252784784220752No Hit
CTCCTCATCTATGCTGCCTCCATCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGTGTCTGGGACAGA4550.12093312531063867No Hit
GCAGAGACCTGGCCAGACTCCCAGACTCCTCATCTATGCTGCCTCCATCAGGGCCACTGGCGTCCCAGACAGGTT4440.11800946733609576No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC4430.11774368024750094No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA4420.11747789315890614No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC4410.11721210607031132No Hit
GAGTGAAGTCTGTCCCAGACACACTGCCACTGAACCTGTCTGGGACGCCA4300.11428844809576841No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4300.11428844809576841No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC4270.11349108682998396No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT4240.11269372556419954No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC4190.1113647901212255No Hit
GACCTGGCCAGACTCCCAGACTCCTCATCTATGCTGCCTCCATCAGGGCC4120.10950428050106181No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4110.109238493412467No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC4080.10844113214668258No Hit
GTGCAGCCACAGTTCTCTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTG4060.10790955796949296No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGCCTCCCAGT4030.10711219670370853No Hit
CAAGAGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT4010.1065806225265189No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC3990.10604904834932928No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT3980.10578326126073447No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC3960.10525168708354485No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA3940.10472011290635523No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC3890.1033911774633812No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGCCTCCCAGTCTCAAAT3870.10285960328619155No Hit
GTCAGACTGTTAGCAGTCGCTACTTAGCCTGGTATCAGCAGAGACCTGGCCAGACTCCCAGACTCCTCATCTATG3820.10153066784321751No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC3810.1012648807546227No Hit
ATATGGAAGCTCACCGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAGAGAACTGTGGCTGCACCAT3800.1009990936660279No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC3770.10020173240024347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)