FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906639
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTATTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT47400.522810071042609No Hit
GTATAATATTGGTGACAGTAGTAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATTTGTC46880.5170746019088082No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36250.3998283771159194No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32400.35736384602912513No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25250.2785011454393645No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGCGTAAAACACT25160.27750846808928364No Hit
GTAGTAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATTTGTCCCAGACCCGTTGCCA23920.2638315801548356No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23910.2637212826714933No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT22850.2520297494372071No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22510.24827963500356812No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21320.23515423448583175No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20050.22114645410135675No Hit
GTTTTACGCAGCTCCGACAATAAGAACTACTTAGCTTGGTACCAGCAGAA19610.2162933648342946No Hit
GTTTACTACTGTCACCAATATTATACTATTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA19240.21221235795062862No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19230.21210206046728633No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18640.2055945089500893No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18600.20515331901672001No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17990.1984251725328383No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17550.19357208326577613No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17520.19324119081574914No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17090.18849839903202928No Hit
GTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGCCCAGCCAGAGTGTTTTACG16850.18585125943181355No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT16800.18529977201510192No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA16330.18011579029801278No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC15900.1753729985142929No Hit
GTCCCTGACCGATTCAGTGGCAACGGGTCTGGGACAAATTTCACTCTCAC15560.17162288408065393No Hit
ATACTATTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA15550.1715125865973116No Hit
CCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGCCCAGC15090.1664389023635648No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14590.16092402819644863No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14370.15849748356291754No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14130.1558503439627018No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14010.15452677416259394No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13740.15154874211235123No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13460.1484604125787662No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTG13170.14526178556183883No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATTTGTCCCAGACCCGTTGCCACTGAATCGGTCAGGGACC13080.14426910821175792No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12610.13908512649466875No Hit
CAGTAGTAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATTTGTCCCAGACCCGTTGCCA12570.1386439365612995No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA12370.13643798689445302No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12060.13301876491084103No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11980.13213638504410247No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11790.13004073286059833No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11770.12982013789391367No Hit
ATTCAGTGGCAACGGGTCTGGGACAAATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA11700.12904805551051743No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT11480.1266215108769863No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11340.1250773461101938No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11330.12496704862685148No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11250.12408466876011288No Hit
CTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGCCCAGCCAGAGTGTTTTACGCAGCTCCGA11170.12320228889337431No Hit
GAATAGTATAATATTGGTGACAGTAGTAAACTGCCACATCTTCAGCCTGC11050.12187871909326645No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGG10900.12022425684313162No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10800.11912128200970838No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGCCTGGAGTGGGTTG10720.11823890214296981No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10660.11757711724291588No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10660.11757711724291588No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10500.1158123575094387No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10460.11537116757606941No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG10340.11404759777596155No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC10250.11305492042588065No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10250.11305492042588065No Hit
GCGTAAAACACTCTGGCTGGGCTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAACCAGGGAGTCTGGAGA10180.1122828380424844No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9990.11018718585898024No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9930.10952540095892631No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC9830.1084224261255031No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9760.10765034374210683No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTACTACTGTCACCAATATTATACTATTCCTCGGACGTT9740.10742974877542219No Hit
ACCATCAACTGCAAGCCCAGCCAGAGTGTTTTACGCAGCTCCGACAATAA9730.10731945129207987No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9590.10577528652528737No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATC9460.10434141924183715No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT9350.10312814692507162No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9290.10246636202501769No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT9260.1021354695749907No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9200.10147368467493677No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9110.10048100732485588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18000.039.097221
CTCTTAT1300.038.8525961
ATCTCGT3300.038.40228737
CGTATGC3550.037.94276441
TCGTATG3650.037.78957740
TTATACA3700.037.28782
TCGCTAA3750.037.02752731
GCTAATC3400.037.00069833
GGTATCA7300.036.9010961
TATACAC3850.036.7090533
CGCTAAT3700.036.68997632
CTAATCT3450.036.5275634
CTTATAC3800.036.330591
ACACTCG3900.036.32658427
ACTCGCT3850.035.9759129
TAATCTC3600.035.06626535
CACGAGA4050.034.9155221
CGAGACA4150.034.8931723
GTATGCC4000.034.62388642
CCCACGA4200.034.4644619
Produced by FastQC (version 0.11.5)