FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906639
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTATTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT46710.5151995446919888No Hit
GTATAATATTGGTGACAGTAGTAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATTTGTC43830.48343386948939987No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34770.3835043495812556No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31870.35151807941198204No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30180.3328778047271296No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24090.26570663737165506No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGCGTAAAACACT23790.2623977128713854No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23350.25754462360432323No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT23040.25412540162071123No Hit
GTAGTAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATTTGTCCCAGACCCGTTGCCA21980.24243386838642503No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21160.23338947475235455No Hit
GTTTACTACTGTCACCAATATTATACTATTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA20280.2236832962182302No Hit
GTTTTACGCAGCTCCGACAATAAGAACTACTTAGCTTGGTACCAGCAGAA19230.21210206046728633No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18670.20592540140011625No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18310.20195469199979266No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18310.20195469199979266No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18270.20151350206642338No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18050.19908695743289226No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17580.1939029757158031No Hit
GTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGCCCAGCCAGAGTGTTTTACG17130.18893958896539859No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16690.1840864996983364No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16550.18254233493154387No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16480.1817702525481476No Hit
CCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGCCCAGC16390.18077757519806673No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15820.17449061864755433No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA15130.16688009229693404No Hit
GTCCCTGACCGATTCAGTGGCAACGGGTCTGGGACAAATTTCACTCTCAC15070.16621830739688012No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC14920.1645638451467453No Hit
ATACTATTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA14870.16401235773003367No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14160.15618123641272877No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14050.15496796409596322No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13700.15110755217898195No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13640.150445767278928No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13140.14493089311181187No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA12690.13996750636140737No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTG12660.13963661391138038No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12560.13853363907795715No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12260.13522471457768748No Hit
ATTCAGTGGCAACGGGTCTGGGACAAATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA11970.13202608756076015No Hit
CAGTAGTAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATTTGTCCCAGACCCGTTGCCA11930.13158489762739084No Hit
CTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGCCCAGCCAGAGTGTTTTACGCAGCTCCGA11450.12629061842695935No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATTTGTCCCAGACCCGTTGCCACTGAATCGGTCAGGGACC11450.12629061842695935No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11410.12584942849359007No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11370.12540823856022076No Hit
GAATAGTATAATATTGGTGACAGTAGTAAACTGCCACATCTTCAGCCTGC10930.12055514929315858No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10780.11890068704302374No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10740.11845949710965445No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA10710.11812860465962748No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT10480.11559176254275408No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10370.11437849022598852No Hit
CTACATGGACTGGGTCCGCCAGGCTCCAGGGAAGGGCCTGGAGTGGGTTG9970.10996659089229562No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGG9950.10974599592561098No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC9950.10974599592561098No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9930.10952540095892631No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGTTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG9920.10941510347558399No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9860.10875331857553006No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9810.10820183115881844No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9800.10809153367547612No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9630.10621647645865663No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9570.1055546915586027No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9530.10511350162523342No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATC9470.10445171672517949No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9310.10268695699170233No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9270.10224576705833302No Hit
GGCTGAAGATGTGGCAGTTTACTACTGTCACCAATATTATACTATTCCTC9160.10103249474156747No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9070.10003981739148658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)