FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269757
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51890.40866086975696925No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51000.4016516546079289No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36650.28863790473295287No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33690.2653263577204142No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32370.2549306678364443No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30690.24169978980230078No Hit
GAATAGTGCTGTAACTCTGTTGACAGTAGTAAGTCGCAAAATCTTCAGCC29820.23484808510604785No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29550.23272169399341766No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29370.23130409991833084No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28470.2242161295428968No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28350.22327106682617226No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27090.2133479083005646No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25540.2011408482095393No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23160.18239710432783596No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23160.18239710432783596No Hit
GGCCAGGGCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGCC22500.177199259385851No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21830.17192265921747232No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21790.17160763831189746No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20950.1649921992948257No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT20620.1623932768238332No Hit
GCACTATTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAAATCAAACGA19000.14963493014805196No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18800.14805982562017772No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18480.14553965837557895No Hit
GTATTATAGTGGGAGCACCTATTACAACCCGTCCCTCAAGAGTCGAGTCACAATGTCCGTAGACACGTCCAAGAA18400.14490961656442924No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG18200.14333451203655503No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18100.14254695977261791No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17400.1370340939250581No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17140.13498645803882162No Hit
CTCCACGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC16850.13270255647340395No Hit
GTAATAAACCCCAACATCCTCAGGCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA16310.12844977424814355No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16000.1260083622299385No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15450.12167682477828436No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC15430.12151931432549694No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15430.12151931432549694No Hit
GTAATAAACAGCCGAGTCTGCGGCGGTCACAGAATTCAGCTTCAGGGAAAACTGGTTCTTGGACGTGTCTACGGA15200.11970794411844156No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15170.11947167843926042No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15150.119314167986473No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15080.11876288140171701No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14880.1171877768738428No Hit
GCTATAGCCTATGTGTCTCACACAGTAATAAACAGCCGAGTCTGCGGCGGTCACAGAATTCAGCTTCAGGGAAAA14820.11671524551548051No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT14380.1132500155541572No Hit
TCCGTGGAGTTTGCAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCA14360.11309250510136978No Hit
GTTTATTACTGTGTGAGACACATAGGCTATAGCAGCGCGTCGGGTTTTGA14280.1124624632902201No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14210.11191117670546413No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14170.11159615579988927No Hit
GGTCTAGTCAGAGCCTCCTGCATACTAATGGATACAACTATTTGGATTGGTACCTGCAGAGGCCAGGGCAGTCTC13950.10986354081922761No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13840.10899723332889678No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13750.10828843629135337No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13730.10813092583856596No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13690.10781590493299112No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13630.10734337357462885No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13550.10671333176347916No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13510.1063983108579043No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13510.1063983108579043No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG13480.10616204517872316No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13430.10576826904675461No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13270.10450818542445522No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13260.10442943019806153No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13170.10372063316051812No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13080.10301183612297471No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13060.10285432567018729No Hit
GTTTAGGGGCTTTCCCTGGTTTTTGCTGGTACCAGTTTAAATAGGTTCTA12990.1023030390854313No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAACTCAGAACATTAGAACCTATTTAAACTGGTACCAGCAAAAA12920.10175175250067532No Hit
GTACCTGCAGAGGCCAGGGCAGTCTCCACATCTCCTGATCTATTTGGGTTCTAGTCGGGCCTCCGGGGTCCCTGA12910.10167299727428161No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12900.1015942420478879No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12850.10120046591591936No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12770.10057042410476966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)