FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004684516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264398
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT32531.2303421357196347No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGC10290.389185999894099No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTGCTGCTGTCCCACAC8610.3256454284828176No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG7990.30219593189055893No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCGCTCTCAGTGT7370.2787464352983003No Hit
CCTATGAGCTGACTCAGCCGCTCTCAGTGTCAGTGACCCTGGGACAGACG7260.27458604074160925No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGG6950.26286129244547995No Hit
GTAATACACGGCCGTGTCCGTGGCGGTCACAGAGCTCAGCGTCAGGGAGAACTGGTTCTTGGGCGTGTCTGCTGA6900.2609702040106204No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6850.2590791155757608No Hit
CAGTAATACACGGCCGTGTCCGTGGCGGTCACAGAGCTCAGCGTCAGGGAGAACTGGTTCTTGGGCGTGTCTG6850.2590791155757608No Hit
GGATTACCTGTGGGGGAAACAACATTGGCAGTTACAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC6010.22730882987012005No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGCAGT5940.22466130606131665No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT5790.21898804075673795No Hit
GCCTATGTGGTCTTCGGCGGAGGGACTAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCT5490.20764151014758056No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT5340.20196824484300185No Hit
GTCTCTGGTGGCTCCATCAATACTTACTACTGGAGCTGGATCCGGCAGCC5210.19705141491236697No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC5110.19326923804264784No Hit
CCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTAGTCCCTC5080.19213458498173208No Hit
CCCCTGTGCTGGTCATCTATAACGATAGCAGTCGGCCCTCTGGGATCCCT5030.1902434965468725No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT4870.1841920135553219No Hit
GTATTACTGTGCGCGAGCGTCCTTCGGTGGCGTCGATGCTTTTGATATATGGGGCCAAGGGACAATGGTCACCGT4860.18381379586834998No Hit
GTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGCGG4850.18343557818137807No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTC4710.17814053056377127No Hit
GCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCGTCATCCC4480.16944152376341726No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4470.16906330607644535No Hit
CTATTACTGTCAGGTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGGCG4410.1667939999546139No Hit
GTGTATTACTGTGCGCGAGCGTCCTTCGGTGGCGTCGATGCTTTTGATAT4330.16376825845883855No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT4330.16376825845883855No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTGTAACTG4310.16301182308489476No Hit
GGACTAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAG4180.15809499315425987No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG4080.15431281628454072No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT3980.1505306394148216No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC3860.14599202717115864No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGG3800.14372272104932715No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA3760.1422098503014395No Hit
CCACATAGGCAGTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCGTCA3730.14107519724052375No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG3700.13994054417960802No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA3690.13956232649263609No Hit
GTCCCACACCTGACAGTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGCGGCCGTGTTCCC3670.13880589111869227No Hit
GGGTATATCTATTTCAGGGGGAGCACCACCTACAAGTCCTCCCTCAAGAGTCGAGTCACCATGTCAGCAGACACG3590.13578014962291696No Hit
CTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC3570.13502371424897314No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT3560.13464549656200123No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG3560.13464549656200123No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC3560.13464549656200123No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC3520.13313262581411356No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA3490.13199797275319783No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG3450.1304851020053102No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC3450.1304851020053102No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG3310.1251900543877034No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGG3170.1198950067700966No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG3130.11838213602220894No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATAACGATAGCAGTCGGCCCTCTGG3110.11762570064826512No Hit
GTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGGCGGAGGGACTAAGCTGACCGTCCTGGGTCAGCCCAAGG2980.11270887071763024No Hit
GTGTCAGTGACCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACAA2950.11157421765671449No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAATACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA2910.11006134690882685No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCGCTCTCAGTGT2880.10892669384791111No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG2850.10779204078699535No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG2840.10741382310002344No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA2830.10703560541305153No Hit
GACCCAGGACGGTCAGCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTG2820.10665738772607962No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT2820.10665738772607962No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGC2810.10627917003910772No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT2780.10514451697819197No Hit
GTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGC2720.10287521085636049No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC2720.10287521085636049No Hit
CAGTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGC2720.10287521085636049No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG2690.10174055779544475No Hit
GTGCTGGTCATCTATAACGATAGCAGTCGGCCCTCTGGGATCCCTGAGCG2690.10174055779544475No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG2660.10060590473452902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)