FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264398
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49211.8612092375887865No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAGC9850.37254442166733487No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTGCTGCTGTCCCACAC8220.31089493869091295No Hit
CTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCGCTCTCAGTGT7820.2957662312120364No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTG7540.28517613597682284No Hit
CCTATGAGCTGACTCAGCCGCTCTCAGTGTCAGTGACCCTGGGACAGACG6960.26323951013245184No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGG6710.253784067958154No Hit
CAGTAATACACGGCCGTGTCCGTGGCGGTCACAGAGCTCAGCGTCAGGGAGAACTGGTTCTTGGGCGTGTCTG6500.24584149653174384No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6440.24357219040991232No Hit
GGATTACCTGTGGGGGAAACAACATTGGCAGTTACAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC6290.23789892510533364No Hit
GTAATACACGGCCGTGTCCGTGGCGGTCACAGAGCTCAGCGTCAGGGAGAACTGGTTCTTGGGCGTGTCTGCTGA6180.23373853054864258No Hit
CCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACAACATTGGCAGT5810.21974447613068177No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT5680.21482764620004688No Hit
GCCTATGTGGTCTTCGGCGGAGGGACTAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCT5390.2038593332778614No Hit
GTCTCTGGTGGCTCCATCAATACTTACTACTGGAGCTGGATCCGGCAGCC5260.19894250334722652No Hit
CCCCTGTGCTGGTCATCTATAACGATAGCAGTCGGCCCTCTGGGATCCCT5260.19894250334722652No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC5180.1959167618514512No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT5040.19062171423384444No Hit
GTATTACTGTGCGCGAGCGTCCTTCGGTGGCGTCGATGCTTTTGATATATGGGGCCAAGGGACAATGGTCACCGT4980.18835240811201295No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4830.18267914280743425No Hit
CCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTAGTCCCTC4760.18003161899863085No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACT4750.17965340131165894No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT4600.17398013600708023No Hit
CTATTACTGTCAGGTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGGCG4590.17360191832010832No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTC4360.1649029115197543No Hit
GTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGCGG4190.15847321084123178No Hit
GTGTATTACTGTGCGCGAGCGTCCTTCGGTGGCGTCGATGCTTTTGATAT4170.15771677546728796No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG4000.15128707478876544No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTGTAACTG3950.14939598635390586No Hit
GGACTAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTCTTCCCGCCCTCCTCTGAGGAG3930.148639550979962No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC3810.14410093873629906No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT3800.14372272104932715No Hit
GCTTAGTCCCTCCGCCGAAGACCACATAGGCAGTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCGTCATCCC3610.1365365849968608No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG3570.13502371424897314No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG3530.13351084350108547No Hit
GGGTATATCTATTTCAGGGGGAGCACCACCTACAAGTCCTCCCTCAAGAGTCGAGTCACCATGTCAGCAGACACG3520.13313262581411356No Hit
CTCTCTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC3510.13275440812714168No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG3490.13199797275319783No Hit
GTCCCACACCTGACAGTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGCGGCCGTGTTCCC3320.1255682720746753No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAAGAGAGTGACCGAGGGGGCAGCCTTGGG3280.12405540132678765No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC3270.12367718363981574No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT3250.12292074826587192No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA3150.11913857139615278No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC3110.11762570064826512No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT3040.11497817683946171No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA3030.1145999591524898No Hit
CCACATAGGCAGTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCGTCA3020.11422174146551789No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA3010.11384352377854598No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG3010.11384352377854598No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGG2990.11308708840460215No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG2980.11270887071763024No Hit
GCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAGAACCTATGAACATTCT2950.11157421765671449No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA2910.11006134690882685No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATAACGATAGCAGTCGGCCCTCTGG2890.10930491153488302No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGGTTCTGTGGCCTCCTATGAGCTGACTCAGCCGCTCTCAGTGT2830.10703560541305153No Hit
GTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGC2790.10552273466516388No Hit
GTGTGGGACAGCAGCACTGCCTATGTGGTCTTCGGCGGAGGGACTAAGCTGACCGTCCTGGGTCAGCCCAAGG2750.10400986391727622No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC2720.10287521085636049No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC2720.10287521085636049No Hit
GTGTCAGTGACCCTGGGACAGACGGCCAGGATTACCTGTGGGGGAAACAA2700.10211877548241666No Hit
CAGTAATAGTCAGCGTCATCCCCGGCTTGGGCTCCGCTGATGGTCAGGGC2690.10174055779544475No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC2670.10098412242150093No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA2650.10022768704755708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA651.8275205E-764.3164970
GTCGAGG200.0056839955.2286561
TGGATTG2650.044.69793370
TACGGAG307.349071E-444.587842
GGTATCA3250.040.167051
GTACGGA350.001561538738.2543371
GTGGTAT1150.037.8385281
GTATCAA7350.036.8881071
TCAGACA651.3920964E-534.30122463
GTCAGAC651.4347492E-534.15046762
TGGTATC1300.033.4408842
CAGACAA702.2522006E-531.97211664
GGTCAGA702.4615938E-531.5592361
TTTATTC752.1240576E-631.2055854
GTCTGCT1900.030.23923967
GGGACTG2200.029.97487660
AACGCAG8850.029.8455376
TGTCTGC1900.029.75566966
CAACGCA8950.029.5120665
TCAACGC8850.029.4677454
Produced by FastQC (version 0.11.5)