FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549452
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18340.33378711880200634No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18150.33032912793110225No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15060.2740912763990303No Hit
GCATTAATGTCGCCCCATCTCACACAATAATAAACAGCCGTGTCCTCGGC14690.26735729417674337No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11480.2089354484104162No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11430.20802545081280988No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10850.1974694786805763No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10450.19018949789972556No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTCGCAACTACTTAGCCTGGTA10400.1892795003021192No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9620.17508353777946026No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCATAGAGCCTGAAGATG9380.17071554931094982No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8960.16307156949105656No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8910.1621615718934502No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8900.16197957237392893No Hit
GTTTATTACTGTCAGCAGCGTGGCAACTGGCCTCCGCTCACTTTCGGCGG8810.1603415766982375No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8730.15888558054206736No Hit
CTCCTGCAGGGCCAGTCAGAGTATTCGCAACTACTTAGCCTGGTACCAACAGAAGCCTGGTCAGGCTCCCAGGCT8710.1585215815030248No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8610.15670158630781214No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8450.15378959399547185No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8300.15105960120265283No Hit
CATTAATGCTGTGCACCTCGCCCTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAGCATCCCCGAC8220.14960360504648268No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8150.14832960840983378No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT8130.14796560937079126No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8050.1465096132146211No Hit
GTCTCCAGTCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTCGCAA7980.14523561657797224No Hit
GCATAGAGCCTGAAGATGTTGCAGTTTATTACTGTCAGCAGCGTGGCAAC7960.1448716175389297No Hit
GTAATAAACTGCAACATCTTCAGGCTCTATGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA7860.14305162234371702No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA7600.13831963483616402No Hit
GACTTCACTCTCACCATCAGCAGCATAGAGCCTGAAGATGTTGCAGTTTATTACTGTCAGCAGCGTGGCAACTGG7550.13740963723855767No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7440.13540764252382373No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7290.1326776497310047No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7180.13067565501627076No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7170.1304936554967495No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT7100.12921965886010062No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6990.12721766414536664No Hit
GTGTTGACACAGTCTCCAGTCACCCTGTCTTTGTCTCCAGGGGAAAGAGC6940.1263076665477603No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTGCACCTTGGTCC6940.1263076665477603No Hit
GCACTGAACACCGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC6730.12248567663781368No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6720.1223036771182924No Hit
ACAGAAGCCTGGTCAGGCTCCCAGGCTCCTCATTTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT6700.12193967807924988No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6650.12102968048164353No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCGAGTGTGAGGTGCATCTGGTGGAGT6640.12084768096212227No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6460.11757168961073942No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6380.11611569345456928No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC6350.11556969489600548No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT6010.10938171123228235No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6010.10938171123228235No Hit
CTCCTGTGCAGGCTCTGGATTCAGCTTCAGCACTTATCAAATGAAATGGGTCCGCCAGGCTCCAGGGAAGGGG6000.10919971171276108No Hit
GTGCAGCCACAGTTCGTTTGACTTGCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGCCAGTTGCCACGCTG5990.10901771219323982No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5980.10883571267371855No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT5970.10865371315419727No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT5950.10828971411515474No Hit
ATAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATCTGCAGATACA5800.10555972132233571No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC5740.1044677242052081No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC5640.10264772900999541No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5630.10246572949047415No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG5600.10191973093191034No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5600.10191973093191034No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5540.10082773381478273No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5540.10082773381478273No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC5530.10064573429526145No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC5520.1004637347757402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5100.052.4293371
GTAACGG200.007552968351.407043
TCGTATG1550.042.3802940
TATCTCG1450.040.3786436
CTTATAC1700.040.3302571
TATACAC1700.040.319253
GTATCAA12550.040.153511
GCTAAGG807.729974E-540.0442370
ATCTCGT1500.039.0612637
CTATCTC1600.038.71735435
CGTATGC1700.038.6550541
ATCTCCG1800.036.1786210
GAGACCG1900.036.101424
GCCTATC1650.035.4130133
AGACCGG1850.035.22967525
ACGAGAC1850.035.21684322
CACGAGA1950.035.16931521
ACATCTC2050.035.1104438
TTATACA2050.035.107252
ATGCCGT1900.034.68164444
Produced by FastQC (version 0.11.5)