FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549452
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18520.3370631101533892No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15700.2857392456483915No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14240.2591673157982863No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14000.2547993273297759No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12180.221675414776905No Hit
GCATTAATGTCGCCCCATCTCACACAATAATAAACAGCCGTGTCCTCGGC12050.2193094210231285No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTCGCAACTACTTAGCCTGGTA10490.19091749597781063No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10420.18964349934116173No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9820.17872352816988563No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9560.17399154066233263No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCATAGAGCCTGAAGATG9190.16725755844004572No Hit
CTCCTGCAGGGCCAGTCAGAGTATTCGCAACTACTTAGCCTGGTACCAACAGAAGCCTGGTCAGGCTCCCAGGCT8950.16288956997153528No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8900.16197957237392893No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8780.1597955781396737No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8400.1528795963978655No Hit
GTTTATTACTGTCAGCAGCGTGGCAACTGGCCTCCGCTCACTTTCGGCGG8080.14705561177318494No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8070.14687361225366363No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7990.14541761609749349No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7920.1441436194608446No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT7840.14268762330467447No Hit
GTAATAAACTGCAACATCTTCAGGCTCTATGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA7660.13941163195329162No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7650.13922963243377037No Hit
GTCTCCAGTCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTCGCAA7510.13668163916047263No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7400.13467964444573866No Hit
GACTTCACTCTCACCATCAGCAGCATAGAGCCTGAAGATGTTGCAGTTTATTACTGTCAGCAGCGTGGCAACTGG7380.13431564540669613No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7320.1332236482895685No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7190.130857654535792No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7120.12958365789914314No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA6900.12557966846967525No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCGAGTGTGAGGTGCATCTGGTGGAGT6800.12375967327446255No Hit
GCATAGAGCCTGAAGATGTTGCAGTTTATTACTGTCAGCAGCGTGGCAAC6790.12357767375494129No Hit
GTGTTGACACAGTCTCCAGTCACCCTGTCTTTGTCTCCAGGGGAAAGAGC6780.12339567423542003No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6650.12102968048164353No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6600.12011968288403718No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT6600.12011968288403718No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6480.11793568864978198No Hit
GTGCAGCCACAGTTCGTTTGACTTGCACCTTGGTCCCTCCGCCGAAAGTGAGCGGAGGCCAGTTGCCACGCTG6480.11793568864978198No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6400.11647969249361181No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTGCACCTTGGTCC6380.11611569345456928No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6280.11429569825935658No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6260.11393169922031407No Hit
ACAGAAGCCTGGTCAGGCTCCCAGGCTCCTCATTTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT6240.11356770018127152No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC6180.11247570306414391No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6180.11247570306414391No Hit
CATTAATGCTGTGCACCTCGCCCTTGACTACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAGCATCCCCGAC6130.11156570546653757No Hit
CTCCTGTGCAGGCTCTGGATTCAGCTTCAGCACTTATCAAATGAAATGGGTCCGCCAGGCTCCAGGGAAGGGG6060.1102917088298887No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5940.10810771459563347No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5880.10701571747850586No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5850.10646971891994206No Hit
GCACTGAACACCGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC5780.10519572228329316No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC5520.1004637347757402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)