FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790617
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATAGTTATCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT40270.5093490274051785No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31270.395513883460639No Hit
CTATCATACTGTTGGCAGTAAAAAGCTGCACAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAACTCTGTC30800.3895691592768686No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30000.37945047981513175No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19990.25284050305014943No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19360.24487204297403167No Hit
GAGTTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTGTGCAGCTTTTTACTGCCAACAGTATGATAGTTAT18580.23500633049883826No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17280.21856347637351586No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17160.21704567445425532No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17140.21679270746771193No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16940.21426303760227772No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16710.21135391725702837No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15860.20060282032893298No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15140.1914960088133698No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG14930.1888398554546639No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14500.18340106524398034No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATCAGTAGCTTATTGGCCTGGTATCAGCAGAAA14230.17998601092564415No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14190.1794800769525573No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT14180.1793535934592856No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA13790.17442073722168888No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13420.1697408479706356No Hit
GAATATCAGTAGCTTATTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTGAACTCCTGATCTATAAGGCGT13150.1663257936522994No Hit
CAGTATGATAGTTATCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT12870.1627842558406915No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12540.15861030056272507No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12510.15823085008290994No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT12470.1577249161098231No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12300.15557469672420401No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12200.1543098617914869No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11670.14760623664808623No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11620.1469738191817277No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11530.1458354677422823No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11120.14064964451814216No Hit
CACCATCACCAGCCTGCAGCCTGATGATTGTGCAGCTTTTTACTGCCAACAGTATGATAGTTATCCGTGGACGTT10900.13786700766616453No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC10720.13559030478727374No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC10660.13483140382764347No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10140.12825426217751454No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9850.12458624087263491No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9760.12344788943318952No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC9670.12230953799374414No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9540.12066525258121188No Hit
GCTTTTTACTGCCAACAGTATGATAGTTATCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA9370.11851503319559281No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9310.11775613223596254No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATCAGTAG9200.11636481380997374No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8990.1137086604512678No Hit
ATCATACTGTTGGCAGTAAAAAGCTGCACAATCATCAGGCTGCAGGCTGG8650.10940822168002964No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8440.10675206832132372No Hit
ATCTGGGACAGAGTTCACTCTCACCATCACCAGCCTGCAGCCTGATGATT8390.10611965085496518No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8350.10561371688187833No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA8310.1051077829087915No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC8210.10384294797607438No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8210.10384294797607438No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC8200.10371646448280267No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8160.10321053050971582No Hit
CTCCTGATCTATAAGGCGTCTTATTTAAAAAGTGGGGTCCCATCAAGGTT7990.10106031112409675No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG7990.10106031112409675No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7980.10093382763082503No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7970.10080734413755332No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7970.10080734413755332No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG7920.10017492667119478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC252.766124E-454.3700371
TCGTATG2500.049.6811140
CGTATGC2700.048.61284641
TATCTCG2400.048.56724536
ACGAGAC2700.047.8406322
GCCTATC2600.047.3023833
TATACAC2900.046.8529363
CACGAGA2800.046.12035821
AGCCTAT2700.045.53016732
CTTATAC3000.044.1756551
ATCTCGT2650.044.0754537
ATGCCGT3000.043.9408944
GAGCCTA2900.043.5510531
GTATGCC3050.043.08974542
TCTCCGA3000.043.03205511
TTATACA3200.042.4604722
GACCGGA3150.041.0530226
AGACCGG3150.041.02962525
ATCTCCG3150.040.9829110
CCCACGA3150.040.98031619
Produced by FastQC (version 0.11.5)