FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624247
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT26680.4273949254061293No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC20530.3288762300820028No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18650.2987599459829202No Hit
GTAATAAACAGCCGTGTCCTCCACTCTCAGGCTGTTCATTTGCAGATACA17270.2766533119101894No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA16910.2708863638912161No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15010.2404496937910795No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTACCA14490.2321196577636737No Hit
ATTATATACTACGCAGACTCTGTGAAGGGCCGAATCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTG13740.22010518272414606No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13180.21113437469463212No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12420.1989597066545774No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12170.19495488164140157No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12090.19367333763718528No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11970.19175102163086086No Hit
GTATATAATACTACCACTACTAGTAATGTATGAAATCCACTCCAGCCCCCTCCCTGGAGGCTGGCGGACCCAGGT11970.19175102163086086No Hit
AGCCAGGACAGGCCCCTGTAGTTGTCATATCTGGTAACAATCACCGGCCC11620.18614426661241462No Hit
GTTTATTACTGTGCGAGACTCACCAGCGTGGATATTTTCAACTACTTTGA11570.18534330160977947No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT10850.17380940557183294No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAG10710.17156670356445444No Hit
CCCCTGTAGTTGTCATATCTGGTAACAATCACCGGCCCTCAGGGATCCCA10700.17140651056392742No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10680.17108612456287334No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10190.16323666753704863No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA10190.16323666753704863No Hit
GGATGAGGCTGACTATTATTGTAACTCCCGGGACAGCAGTGGTAACACTTGGGTGTTCGGCGGAGGGACCAAG9940.15923184252387276No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT9710.15554740351175098No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9640.1544260525080617No Hit
CTATTATTGTAACTCCCGGGACAGCAGTGGTAACACTTGGGTGTTCGGCG9380.1502610344943588No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG9360.14994064849330474No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG9330.14946006949172363No Hit
CTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTT9220.14769794648592624No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9130.14625620948118292No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA8910.14273196346958816No Hit
GTGTTACCACTGCTGTCCCGGGAGTTACAATAATAGTCAGCCTCATCCTC8790.14080964746326374No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTGGTA8730.13984848946010153No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT8660.13872713845641227No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8630.1382465594548312No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8630.1382465594548312No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8550.1369650154506149No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8540.13680482245008785No Hit
GCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGACCGAGAGTCAGAGAATCGGT8490.1360038574474527No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8480.13584366444692567No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7740.12398938240792508No Hit
CAATAATAGTCAGCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGA7690.1231884174052899No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7680.12302822440476285No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7680.12302822440476285No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7670.12286803140423583No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7560.12110590839843843No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTACCAC7460.11950397839316808No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7410.11870301339053291No Hit
GTGGTAACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA7160.11469818837735704No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC7080.11341664437314075No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTAGTTGTCATATCTGGTAACAATCACCGGCC7040.11277587237103262No Hit
GCCTGAGAGTGGAGGACACGGCTGTTTATTACTGTGCGAGACTCACCAGC6940.11117394236576227No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCACCTGGTGGAGT6880.11021278436260007No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAG6800.10893124035838378No Hit
GTATTATATACTACGCAGACTCTGTGAAGGGCCGAATCACCATCTCCAGAGACAACGCCAAGAACTCACTGTAT6770.10845066135680267No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6720.10764969635416749No Hit
GCACTGAACACAGAGGACTAACCATGGAGTTGGGGCTGTGCTGGGTTTTC6720.10764969635416749No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6640.10636815234995123No Hit
CTCCTGTGCAGCCTCTGGATTCACCCTCAGTACATATGAAATGACCTGGGTCCGCCAGCCTCCAGGGAGGGGG6550.1049264153452079No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6550.1049264153452079No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA6540.10476622234468087No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6540.10476622234468087No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6490.1039652573420457No Hit
CTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTCTG6470.10364487134099164No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC6360.10188274833519424No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6350.10172255533466722No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6300.10092159033203205No Hit
CACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTT6290.10076139733150499No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6260.10028081832992389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4400.089.2249770
GTCGTAT253.1443014E-668.8079139
ATCGTAT309.272824E-657.33992839
TATCGTA309.4178085E-657.1900238
GATATCG309.694721E-656.9110436
GGTATCA5000.050.493331
TCGACAC200.0081212250.47310323
TAGGTTG200.00812636650.4650154
CACGCCA200.00812636650.46501516
CGATCAT200.00812636650.46501513
TATAATC200.00812636650.4650157
AAAGGGG4850.048.19204768
CTTATAC5400.047.999831
TCGTATG5300.047.4916240
CGTATGC5400.047.37122341
ATGCCGT5650.046.88079544
TATACAC5600.046.2595943
GCCGTCT5600.046.20802746
ATCTCCG5700.044.8577910
AAAAGGG5000.044.77406767
Produced by FastQC (version 0.11.5)