FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624247
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51990.8328434097400549No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC19750.31638117604089405No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18030.28882797995024406No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA16640.26656115287698623No Hit
GTAATAAACAGCCGTGTCCTCCACTCTCAGGCTGTTCATTTGCAGATACA16220.2598330468548507No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14090.22571193774259227No Hit
ATTATATACTACGCAGACTCTGTGAAGGGCCGAATCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTG13960.2236294287357408No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12820.20536742667565883No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTACCA12500.20024125065879372No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12160.19479468864087454No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12040.19287237263455012No Hit
CCCCTGTAGTTGTCATATCTGGTAACAATCACCGGCCCTCAGGGATCCCA11920.1909500566282257No Hit
GTATATAATACTACCACTACTAGTAATGTATGAAATCCACTCCAGCCCCCTCCCTGGAGGCTGGCGGACCCAGGT11500.1842219506060902No Hit
GTTTATTACTGTGCGAGACTCACCAGCGTGGATATTTTCAACTACTTTGA11150.178615195587644No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC11040.1768530725818466No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT11010.17637249358026552No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10950.17541133557710328No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGA10840.17364921257130592No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10410.16676091354864342No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT10320.16531917654390008No Hit
AGCCAGGACAGGCCCCTGTAGTTGTCATATCTGGTAACAATCACCGGCCC10200.16339686053757566No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAG10080.16147454453125124No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG10020.16051338652808905No Hit
GGATGAGGCTGACTATTATTGTAACTCCCGGGACAGCAGTGGTAACACTTGGGTGTTCGGCGGAGGGACCAAG9860.15795029851965647No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9630.1542658595075347No Hit
CTATTATTGTAACTCCCGGGACAGCAGTGGTAACACTTGGGTGTTCGGCG9300.14897949049014253No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9020.14449408647538553No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG8790.14080964746326374No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA8780.1406494544627367No Hit
GTGTTACCACTGCTGTCCCGGGAGTTACAATAATAGTCAGCCTCATCCTC8630.1382465594548312No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8620.13808636645430414No Hit
CTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTT8520.1364844364490338No Hit
GTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGT8470.1356834714463986No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGAAGCTATTATGCAAGCTGGTA8430.13504269944429048No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8310.13312038343796606No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8120.1300767164279524No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7860.12591169841424948No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG7850.12575150541372246No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7800.12495054041108727No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7600.12174668040054659No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC7590.12158648740001954No Hit
GTGGTAACACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA7520.1204651363963303No Hit
GCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGAAGCTGTGTTTCCTGACCGAGAGTCAGAGAATCGGT7470.11966417139369512No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTAGTTGTCATATCTGGTAACAATCACCGGCC7380.11822243438895182No Hit
CAATAATAGTCAGCCTCATCCTCCGCCTGAGCCCCAGTGATGGTCAAGGA7300.11694089038473553No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7290.11678069738420849No Hit
GCCTGAGAGTGGAGGACACGGCTGTTTATTACTGTGCGAGACTCACCAGC7260.1163001183826274No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7240.11597973238157333No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCACCTGGTGGAGT6770.10845066135680267No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6760.10829046835627565No Hit
GCACTGAACACAGAGGACTAACCATGGAGTTGGGGCTGTGCTGGGTTTTC6750.10813027535574861No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6720.10764969635416749No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6660.1066885383510053No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC6610.10588757334837012No Hit
GTATTATATACTACGCAGACTCTGTGAAGGGCCGAATCACCATCTCCAGAGACAACGCCAAGAACTCACTGTAT6610.10588757334837012No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTACCAC6610.10588757334837012No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6520.10444583634362681No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC6400.10252352033730239No Hit
CTCCTGTGCAGCCTCTGGATTCACCCTCAGTACATATGAAATGACCTGGGTCCGCCAGCCTCCAGGGAGGGGG6350.10172255533466722No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT6330.10140216933361312No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA6320.10124197633308611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAGA350.004631596658.1650470
ATGTAAT200.00815450250.42091
GGTATCA6700.045.6547431
GGAAACG650.00101498441.75951870
GTGGTAT1700.041.5230941
GGTTAGG350.001153438840.6941152
TCTGGAG7950.038.41087370
TGGTATC1850.038.1227232
TAACGGC451.0653674E-437.9298736
GTATCAA17300.037.1113361
TATCAAC18900.034.1173862
TTAACGG502.0121745E-434.04260335
ATGGCAA1201.5013371E-533.92960770
GTGCCAA502.1738272E-433.59776320
ATCAACG19850.032.6459053
TCAACGC20000.032.4010624
CAACGCA19950.032.319145
AACGCAG20100.032.0779576
CGGTACC553.016402E-431.77371645
AACCGCA450.003958991731.65097852
Produced by FastQC (version 0.11.5)