FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004684529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194420
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50120.4196178898544901No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41090.34401634266003583No Hit
GAGTTACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT40310.33748597645719264No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37600.3147971400344937No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34910.2922757488990472No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32550.27251720500326515No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32520.2722660370723866No Hit
GTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTTCGGCCAAGGGG29080.2434654476649755No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28400.2377723078983942No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28170.23584668709499174No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27760.23241405870631773No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTC25960.2173439828536026No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25830.21625558848646204No Hit
GATCTATTCTGCTTCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCA25700.2151671941193215No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTAGTAACTATTTAAATTGGTATCAGCAGAAA23890.20001339562298018No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23470.19649704459068001No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22880.19155740861673448No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22720.19021784631871536No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22710.19013412367508917No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22170.18561310091927463No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21640.18117580080708628No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21090.17657105540764556No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGACCATTAGTAA20910.17506404782237406No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20880.17481287989149547No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20810.1742268213861121No Hit
ACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGA20410.17087791564106428No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20350.17037557977930712No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCCCTTGGCCGAACGTC20200.16911974012491418No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19300.16158470219855664No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19080.15974280403878033No Hit
ACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAATCAAACGAA18370.15379849634132048No Hit
GTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTA18290.15312871519231092No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18260.15287754726143232No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17680.148021633931113No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17450.1460960131277105No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17030.14257966209541031No Hit
GAATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT17020.14249593945178413No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGCCC16670.1395656469248673No Hit
GATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATC16630.13923075635036253No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA16270.1362167411798195No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16160.13529579209993134No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16130.13504462416905275No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15260.1277607541735738No Hit
GGATAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGTTGATGGTGAGAGTGAAAT15160.12692352773731183No Hit
CACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTATCCCCCGGACGTT15060.1260863013010499No Hit
GTGGGGTCCCATCAAGGTTCAGTGGTAGTGGATCTGGGACAGATTTCACT14910.12483046164665697No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTG14820.12407695785402119No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14810.123993235210395No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14670.12282111819962828No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14650.12265367291237589No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14230.1191373218800757No Hit
GTTGTAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTACCA14190.11880243130557089No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13860.11603958406590648No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGACAAGTCAGA13770.1152860802732707No Hit
GTTCAGTGGTAGTGGATCTGGGACAGATTTCACTCTCACCATCAACAGTCTACAACCTGAAGATTTTGCAACTTA13570.11361162740074682No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC13400.11218834245910149No Hit
GAGTGAAATCTGTCCCAGATCCACTACCACTGAACCTTGATGGGACCCCA13280.11118367073558716No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13250.11093250280470857No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13110.10976038579394182No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12910.10808593292141794No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12890.10791848763416555No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGTTACTA12790.10708126119790358No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12730.10657892533614641No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12670.10607658947438925No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12530.10490447246362253No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12440.10415096867098675No Hit
AGTCAGACCATTAGTAACTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATT12360.1034811875219772No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12230.10239279315483667No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12120.1014718440749485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)