FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041439
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35300.3389540818041191No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27830.2672264050030775No Hit
ATTATAATTCTCCGTGGGCCTTCGGCCGAGGGACCAAGGTGGAAGTCAGA24970.23976440290789958No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTTCAGCCCGATGATT24900.23909225600347211No Hit
CTATTATAATTCTCCGTGGGCCTTCGGCCGAGGGACCAAGGTGGAAGTCAGACGAACTGTGGCTGCACCAT23520.22584135988761703No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT22750.21844774393891528No Hit
GAGTTCACTCTCACCATCAGCAGCCTTCAGCCCGATGATTTTGCGACTTATTTCTGCCAACACTATTATAATTCT21550.2069252255773022No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21270.20423663795959246No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21030.20193213428726983No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19280.18512846167658403No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19140.18378416786772916No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18260.1753343210692129No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16430.15776248056775288No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15590.1496967177146237No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15420.14806436094672853No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15120.14518373135632523No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14880.1428792276840026No Hit
GTAGTGGTAGTGGCCGTACTGTGAGATATGCAGACTCACTGGAGGGCCGA14490.13913440921647835No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14260.1369259265305025No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12950.12434717731907485No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12880.12367503041464743No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12860.12348298844195386No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12760.12252277857848612No Hit
GAATTATAATAGTGTTGGCAGAAATAAGTCGCAAAATCATCGGGCTGAAGGCTGCTGATGGTGAGAGTGAACT12750.12242675759213933No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC12640.1213705267423248No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12620.12117848476963125No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12610.12108246378328448No Hit
GTCCCAGACCCAGTGCCGCTGAACCTCGATGGGACCCCTGTTTCTAATCT12530.12031429589251026No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12310.1182018341928812No Hit
GTTTAGGGGCCGTCCCTACTTTCACTTGAAACCAGGCCAACCACGTAAAA12270.11781775024749408No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12190.11704958235671989No Hit
CCATTAACATCACTTGCCGGGCCAGTCAGAGTGTTTTTACGTGGTTGGCCTGGTTTCAAGTGAAAGTAGGGACGG12040.11560926756151824No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11900.11426497375266338No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11570.11109628120321978No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11480.1102320923260988No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11450.10994402936705848No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11310.1085997355582036No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11020.1058151269541471No Hit
CAGTAGTGGTAGTGGCCGTACTGTGAGATATGCAGACTCACTGGAGGGCCGATTCACCGTCTCCAGAGACAACGC11010.10571910596780033No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10890.10456685413163902No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10750.10322256032278415No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10740.10312653933643738No Hit
GTGCTGGGTTCTCCTTGTTGCCATTTTAGAAGGTGTCCAGTGTGAGGTGC10720.10293449736374383No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10650.10226235045931639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)