FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004684531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041439
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43110.4139464721409511No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30800.29574463794806993No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27040.25964074708168217No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTTCAGCCCGATGATT25130.24130073868944796No Hit
ATTATAATTCTCCGTGGGCCTTCGGCCGAGGGACCAAGGTGGAAGTCAGA24810.23822806712635114No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23160.2223846043791331No Hit
CTATTATAATTCTCCGTGGGCCTTCGGCCGAGGGACCAAGGTGGAAGTCAGACGAACTGTGGCTGCACCAT21980.21105412799021353No Hit
GAGTTCACTCTCACCATCAGCAGCCTTCAGCCCGATGATTTTGCGACTTATTTCTGCCAACACTATTATAATTCT21750.2088456453042377No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18780.18032741235924524No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17080.16400384468029333No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16070.15430572505926896No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15600.14979273870097048No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14480.1390383882301316No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14300.13731001047588962No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14240.13673388455780897No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14100.1353895907489541No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14070.13510152778991377No Hit
GTAGTGGTAGTGGCCGTACTGTGAGATATGCAGACTCACTGGAGGGCCGA14000.13442938088548634No Hit
GTCCCAGACCCAGTGCCGCTGAACCTCGATGGGACCCCTGTTTCTAATCT13840.1328930451039379No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13460.12924424762276043No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13240.12713178592313135No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12900.12386707238734099No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12600.1209864427969377No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12350.1185859181382683No Hit
GAATTATAATAGTGTTGGCAGAAATAAGTCGCAAAATCATCGGGCTGAAGGCTGCTGATGGTGAGAGTGAACT12170.11685754038402633No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12060.11580130953421179No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11970.11493712065709082No Hit
GTGCTGGGTTCTCCTTGTTGCCATTTTAGAAGGTGTCCAGTGTGAGGTGC11850.1137848688209295No Hit
GTTTAGGGGCCGTCCCTACTTTCACTTGAAACCAGGCCAACCACGTAAAA11640.11176842810764721No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11630.11167240712130044No Hit
CCATTAACATCACTTGCCGGGCCAGTCAGAGTGTTTTTACGTGGTTGGCCTGGTTTCAAGTGAAAGTAGGGACGG11550.11090423923052622No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11510.11052015528513913No Hit
GAGTTGGGGCTGTGCTGGGTTCTCCTTGTTGCCATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAAT11390.10936790344897782No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11360.10907984048993749No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11040.10600716892684066No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10850.1041827701862519No Hit
CAGTAGTGGTAGTGGCCGTACTGTGAGATATGCAGACTCACTGGAGGGCCGATTCACCGTCTCCAGAGACAACGC10760.10331858130913092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)