FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004684532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930589
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC24530.2635964964124871No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT21860.2349049902803493No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC19780.2125535547916427No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19520.2097596253555544No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACG19310.20750299004179074No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC19120.20546127237695694No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG18740.2013778370472894No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17970.19310350756348935No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17570.18880515458489194No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17040.18310983688825036No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT15710.1688178132344139No Hit
GTACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGGCCCCAGGAAAGGCC15630.16795814263869444No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT15450.16602388379832558No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14870.15979127197935933No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14240.15302136603806837No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTACAGTGTTCCTGTCCCACGTCTGACAGTAATAGTCAG14200.15259153074020862No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14160.15216169544234887No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14010.15054981307537485No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGACGTGGGACAGGAACACTGTAGTCTTCGGAACTGGGACCAAGGT13970.1501199777775151No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13920.14958268365519042No Hit
CCCCAGGACAGACGGCCACCATCACCTGCTCTGGAGATAGATTGGGGGAT13780.14807826011268133No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG12930.1389442600331618No Hit
GACTATTACTGTCAGACGTGGGACAGGAACACTGTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG12820.1377622129640475No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACGGCCACCATCACCTGCTCTGG12700.13647270707046827No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTACAGTGTTCCTGTCCCACGTCTGACAGTAATAGTCAGC12270.13185197761847603No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12260.13174451879401108No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC12140.13045501290043188No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAC11950.1284132952355981No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG11890.1277685422888085No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11430.12282543636342146No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCA11340.12185830694323703No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10580.11369143628390192No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTACAGTGTTC10570.11358397745943698No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10540.11326160098604217No Hit
CTATTACTGTCAGACGTGGGACAGGAACACTGTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC10460.11240193039032267No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG10220.10982291860316423No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10120.10874833035851487No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG9890.10627677739582135No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9850.10584694209796162No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG9640.10359030678419795No Hit
GGCCAGTCCCCTGTGATGCTCATCTATCAAGATACCAAGCGGCCCTCAGG9580.10294555383740835No Hit
CCCCTGTGATGCTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT9560.10273063618847847No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9540.10251571853954862No Hit
CAGCCACTCTGACCATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAGACGTGGGACAGGAACA9540.10251571853954862No Hit
ATCAAGATACCAAGCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAGCCACTCTGA9440.10144113029489925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)