FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004684533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930589
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31660.34021463825598625No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC24750.26596059055071575No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT21200.2278127078656636No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC19140.20567619002588683No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18390.19761677819101664No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18100.19450047228153353No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17240.18525901337754905No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACG17000.1826800015903906No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG16610.1784891074362581No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15580.16742084851636974No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15050.16172553081972815No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14930.16043602492614892No Hit
GTACTAGTGGAGTGGGTGTGGGCTGGATCCGTCAGGCCCCAGGAAAGGCC14490.15570783664969176No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTACAGTGTTCCTGTCCCACGTCTGACAGTAATAGTCAG14060.15108710719769952No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC13760.14786334246375143No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG13730.14754096599035665No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13600.1461440012723125No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA13560.14571416597445275No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13140.14120089534692545No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG12510.1344309894056345No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTAC12160.13066993054936174No Hit
GACTATTACTGTCAGACGTGGGACAGGAACACTGTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG12080.12981025995364226No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGACGTGGGACAGGAACACTGTAGTCTTCGGAACTGGGACCAAGGT12020.12916550700685264No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC11950.1284132952355981No Hit
CCCCAGGACAGACGGCCACCATCACCTGCTCTGGAGATAGATTGGGGGAT11850.12733870699094874No Hit
CCCTCAGTGTCCGTGTCCCCAGGACAGACGGCCACCATCACCTGCTCTGG11500.123577648134676No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATC11380.12228814224109677No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11080.1190643775071487No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG10790.11594807159766557No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTACAGTGTTCCTGTCCCACGTCTGACAGTAATAGTCAGC10650.11444364805515647No Hit
CTATTACTGTCAGACGTGGGACAGGAACACTGTAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC10480.11261684803925255No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG10400.11175717744353307No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTACAGTGTTC10220.10982291860316423No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCA10210.1097154597786993No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGG10140.10896324800744475No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10130.10885578918297982No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10050.10799611858726033No Hit
CCCCTGTGATGCTCATCTATCAAGATACCAAGCGGCCCTCAGGGATCCCT9820.1055245656245668No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9520.10230080089061874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)