FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences845907
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33590.3970885688379455No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22820.2697696082429865No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGACGTCAACTCCAGGGACA22520.2662231190899236No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21080.24919997115522155No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19510.23064001125419226No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18840.22271951881235172No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18480.2184637318286762No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17080.20191344911438253No Hit
GAGTTACGTTACCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT17070.20179523280928047No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAACTATTTAAATTGGTATCAGCAGAAA16860.1993126904021364No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16740.19789409474091124No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16670.19706658060519655No Hit
GATCTATTCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA15410.18217132616233225No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14670.17342331958477705No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14040.16597569236334492No Hit
GTCCCTAACCTCCTGATCTATTCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGAT13820.16337493365109876No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13580.16053774232864843No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13530.15994666080313794No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13140.15533622490415613No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13010.15379941293782887No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12800.15131687053068482No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12790.1511986542255827No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGCCCCTTGGCCGAACGTC12670.14978005856435753No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12530.14812503029292817No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC12430.14694286724190722No Hit
GTCTCTGGTGGCTCCATCAGCAATAATAATTGGTGGAGTTGGGTCCGCCA12400.1465882183266009No Hit
GTGTATTACTGTGCGAGACGTAGTGGGACCTTCCCGTACTGGTACTTCGA12380.1463517857163967No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11850.14008632154598555No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11650.1377219954439436No Hit
CAGTAATACACGGCCGTGTCCGCGGCGGTCACAGACGTCAACTCCAGGGACAACCGATTCTTGGTCTTGTCTA11440.13523945303679955No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT11290.13346620846026808No Hit
GTCCTGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC11100.13122009866332823No Hit
CACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACGTTACCCCCCGGACGTT11100.13122009866332823No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11100.13122009866332823No Hit
GAATAGATCAGGAGGTTAGGGACTTTCCCTGGTTTCTGCTGATACCAATT10830.1280282584255716No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTG10770.12731896059495904No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10760.12720074428985692No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10670.12613679754393806No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGCCC10660.12601858123883594No Hit
CTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT10660.12601858123883594No Hit
ACTTACTACTGTCAACAGAGTTACGTTACCCCCCGGACGTTCGGCCAAGGGGCCAAGGTGGAAGTCAAACGAA10600.12530928340822337No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10580.12507285079801916No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10440.1234178225265898No Hit
ATTCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA10330.12211744317046673No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10250.12117171272964995No Hit
ACGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTC10090.1192802518480164No Hit
GATTTCACTCTCACCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACGTTACC10040.1186891703225059No Hit
CCATCAACAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG9990.11809808879699542No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9930.11738879096638284No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9840.11632484422046394No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA9830.11620662791536186No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA9460.11183262462658426No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9390.11100511049086956No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9360.11065046157556328No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCGTCC9320.11017759635515488No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9280.10970473113474648No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9140.10804970286331712No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9060.10710397242250035No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9020.10663110720209196No Hit
GTCCCTCACCTGCGCTGTCTCTGGTGGCTCCATCAGCAATAATAATTGGT8810.1041485647949479No Hit
GTATTACTGTGCGAGACGTAGTGGGACCTTCCCGTACTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCA8670.10249353652351854No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8630.10202067130311014No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8540.10095672455719126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT200.00773799351.0956888
GTGGTAT2050.046.5481761
GTATAGG651.2676537E-841.9445081
TACTGAT9850.037.85947870
GTATCAA19650.036.5947151
CCGAAAC551.6679839E-435.14811768
TATGCGG400.002763063934.06379310
CTATACC601.46668335E-534.0557374
TATCAAC23200.031.1254182
ATCAACG23850.030.414563
TCAACGC24100.030.0990584
AACGCAG24250.030.0604216
TGTCTAC7150.030.02770466
CAACGCA24350.029.7953155
TCTACTG7750.028.9348468
CGAAACC755.5344735E-428.64732669
TTAACGG605.5511197E-428.63365735
CGGTACT500.00672128228.40475859
GTCTACT7750.028.0663767
ACGCAGA25900.028.0138557
Produced by FastQC (version 0.11.5)