FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611453
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29230.4780416483360127No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28340.4634861551092234No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20750.3393556005122225No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20140.32937936358150177No Hit
GAGTAGGAGGTCAGGGGCTTTCCCTGATTTTTGCTGATACCAGGCTAAAT19920.3257813764917336No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC17900.2927453132129534No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16720.2734470188223788No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16430.26870421765859354No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16250.26576041003969236No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15880.2597092499341732No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15440.25251327575463695No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15100.24695275025226798No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14470.23664942358611374No Hit
GTAATATGTAGCCGTGTCCTCGGCTCGCAGGCTGCTCATTTGCAAATACA14250.23305143649634558No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13730.22454710337507544No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13680.22372937903649176No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT13520.22111266115302403No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTGTGAGAGTGA12830.2098280652805694No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12820.20966452041285263No Hit
GTCTACAACTTGATAGTTACCCTTTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAAGGAACTGTGGCTGCAC12790.20917388580970245No Hit
AGTCAGGACATTGGCAGTTATTTAGCCTGGTATCAGCAAAAATCAGGGAAAGCCCCTGACCTCCTACTCTTTG12670.20721134739710165No Hit
ACATATTACTGTGCGAGAGATTTCACTGGAGTCAAGACTGGGGAAAGAAA12470.20394045004276695No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12340.20181436676244943No Hit
GTAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGGACATTGGCAG12250.20034246295299882No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12200.19952473861441516No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12170.19903410401126498No Hit
ACTTGATAGTTACCCTTTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAAGGAACTGTGGCTGCACCAT12150.1987070142758315No Hit
ACTTATTACTGTCTACAACTTGATAGTTACCCTTTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAAGGAA12120.1982163796726813No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11650.19052977088999482No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11280.18447861078447567No Hit
GAATTCACTCTCACAATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAACTTGATAGTTAC11180.18284316210730833No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCCTGGTCC11070.18104416856242425No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCCGTCTCCATCCTTC10930.17875454041438998No Hit
CTATCAAGTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTGTGAGAGTGAATTCTGTC10420.17041375216083654No Hit
CTCCTACTCTTTGCTGCATCCACTTTGCAAAGTGGGGTCCCATCGAGGTT10380.16975957268996963No Hit
GTGGGGTCCCATCGAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACT10360.16943248295453614No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10220.1671428548065019No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10210.16697930993878515No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10020.1638719574521672No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG9970.16305423311358355No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9740.15929270115609867No Hit
GTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTGTGAGAGTGAATTCTGTCCCAGATC9550.15618534866948072No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9520.15569471406633054No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9100.1488258296222277No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9080.14849873988679424No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8940.14620911173875997No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8820.14424657332615917No Hit
CTCCTGTGCAGCGTCTGGATTTACCTTCAGTAGGTTTGCCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG8480.1386860478237902No Hit
GGGTGGAGATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG8340.13639641967575594No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8340.13639641967575594No Hit
GGACATTGGCAGTTATTTAGCCTGGTATCAGCAAAAATCAGGGAAAGCCCCTGACCTCCTACTCTTTGCTGCATC8290.1355786953371723No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8130.13296197745370453No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8070.13198070824740413No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC7900.12920044549621967No Hit
GACCAGGGTGGAGATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCT7770.12707436221590213No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT7760.12691081734818538No Hit
CAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTGTGAGAGT7740.1265837276127519No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC7620.12462118920015111No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT7590.12413055459700091No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7580.12396700972928418No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7530.12314928539070051No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7510.12282219565526704No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7460.12200447131668338No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7430.12151383671353319No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC7350.1202054777717993No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT7340.12004193290408258No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTGAAAGGGTAACTATCAAGTTGTAGACAGTAATAAGTTGCAAAATCTT7330.11987838803636584No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7260.11873357396234871No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC7260.11873357396234871No Hit
CCTCCTACTCTTTGCTGCATCCACTTTGCAAAGTGGGGTCCCATCGAGGT7240.11840648422691523No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7210.11791584962376504No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7140.11677103554974788No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT7050.1152991317402973No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT7020.11480849713714708No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC7000.11448140740171363No Hit
CTCACAATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCT6980.11415431766628016No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC6940.11350013819541323No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6890.11268241385682955No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC6880.11251886898911283No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG6880.11251886898911283No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6810.1113740549150957No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCG6770.11071987544422875No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6760.11055633057651201No Hit
GATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGGTGAACCTCGATG6750.1103927857087953No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC6710.10973860623792835No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6700.10957506137021161No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC6600.10793961269304428No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6590.10777606782532755No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT6500.10630416401587693No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC6470.10581352941272675No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTT6430.1051593499418598No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC6420.10499580507414308No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6370.1041780807355594No Hit
TATTTAGCCTGGTATCAGCAAAAATCAGGGAAAGCCCCTGACCTCCTACT6350.10385099100012592No Hit
CACCATCACTTGCCGGGCCAGTCAGGACATTGGCAGTTATTTAGCCTGGTATCAGCAAAAATCAGGGAAAGCCC6320.10336035639697573No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6310.103196811529259No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCCGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGA6210.10156136285209166No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGC150.002426974268.4782512
GCCCTAG200.006488535653.42072364
CAACGGG200.007581528751.3586856
TATTCGG200.007581528751.35868511
GTATCAA12000.042.2386171
TATCTCG1250.041.32010736
ATCTCGT1300.039.773437
TCGTATG1150.039.0790140
CGTATGC1200.037.4816741
ACCGATC1500.036.58156227
GACCGAT1500.036.57557326
CTTATAC1500.036.5306971
TTATACA1500.036.5187452
TATCAAC13800.036.4691282
CCGTCTT1350.036.08864647
ATCAACG13900.035.9604533
TCAACGC14050.035.820214
TATACAC1550.035.340723
CAACGCA14300.035.1939855
GTATCTC1500.034.4051335
Produced by FastQC (version 0.11.5)