FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004684539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611453
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29800.4873637057958666No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23010.37631674061620435No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19370.31678640876731323No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18950.30991752432321046No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18500.30255800527595744No Hit
GAGTAGGAGGTCAGGGGCTTTCCCTGATTTTTGCTGATACCAGGCTAAAT17130.2801523583987649No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15980.2613446986113405No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15440.25251327575463695No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC15130.24744338485541817No Hit
GTAATATGTAGCCGTGTCCTCGGCTCGCAGGCTGCTCATTTGCAAATACA15080.24662566051683446No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14780.2417193144853325No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14400.23550460951209656No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14020.22928990453886072No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13880.22700027639082646No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13210.21604277025380528No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13120.21457086644435466No Hit
ACTTATTACTGTCTACAACTTGATAGTTACCCTTTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAAGGAA12980.2122812382963204No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTGTGAGAGTGA12840.20999161014828616No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT12580.20573944358765103No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12550.20524880898450085No Hit
GTCTACAACTTGATAGTTACCCTTTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAAGGAACTGTGGCTGCAC12550.20524880898450085No Hit
ACATATTACTGTGCGAGAGATTTCACTGGAGTCAAGACTGGGGAAAGAAA12250.20034246295299882No Hit
ACTTGATAGTTACCCTTTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAAGGAACTGTGGCTGCACCAT12230.20001537321756535No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12090.1977257450695311No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12080.19756220020181436No Hit
AGTCAGGACATTGGCAGTTATTTAGCCTGGTATCAGCAAAAATCAGGGAAAGCCCCTGACCTCCTACTCTTTG12030.19674447586323068No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11770.19249230930259562No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11360.18578696972620953No Hit
CTCCTACTCTTTGCTGCATCCACTTTGCAAAGTGGGGTCCCATCGAGGTT11230.183660886445892No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11130.18202543776872465No Hit
GTGGGGTCCCATCGAGGTTCACCGGCAGTGGATCTGGGACAGAATTCACT11090.18137125829785772No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11050.18071707882699078No Hit
GAATTCACTCTCACAATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAACTTGATAGTTAC11000.17989935448840713No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCCGTCTCCATCCTTC10500.17172211110257043No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10240.16746994454193534No Hit
GTAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGGACATTGGCAG9780.1599468806269656No Hit
GTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTGTGAGAGTGAATTCTGTCCCAGATC9770.15978333575924888No Hit
CTATCAAGTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTGTGAGAGTGAATTCTGTC9650.15782079734664806No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9620.15733016274349787No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9370.15324154105057952No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9300.1520967269765624No Hit
GTGCAGCCACAGTTCCTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTG9110.14898937448994443No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9030.1476810155482106No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9010.14735392581277712No Hit
GGGTGGAGATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9000.14719038094506037No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8790.14375593872300896No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCCTGGTCC8770.1434288489875755No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA8140.13312552232142127No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8090.1323077979828376No Hit
CTCCTGTGCAGCGTCTGGATTTACCTTCAGTAGGTTTGCCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG7930.12969108009936986No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7910.1293639903639364No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC7760.12691081734818538No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCAAACCTAC7620.12462118920015111No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7610.12445764433243439No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTGAAAGGGTAACTATCAAGTTGTAGACAGTAATAAGTTGCAAAATCTT7550.12347637512613399No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7550.12347637512613399No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC7550.12347637512613399No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7480.12233156105211683No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC7310.11955129830093236No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC7290.1192242085654989No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA7280.11906066369778218No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7260.11873357396234871No Hit
CACCATCACTTGCCGGGCCAGTCAGGACATTGGCAGTTATTTAGCCTGGTATCAGCAAAAATCAGGGAAAGCCC7220.11807939449148178No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6970.11399077279856341No Hit
CTCACAATCAGCGGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCT6960.11382722793084668No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC6930.1133365933276965No Hit
CCTCCTACTCTTTGCTGCATCCACTTTGCAAAGTGGGGTCCCATCGAGGT6920.11317304845997976No Hit
GAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTC6910.11300950359226301No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6850.11202823438596261No Hit
CAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCCGCTGATTGTGAGAGT6840.11186468951824588No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6840.11186468951824588No Hit
GATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGGTGAACCTCGATG6790.11104696517966221No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA6780.11088342031194548No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6770.11071987544422875No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC6750.1103927857087953No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT6620.10826670242847773No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6600.10793961269304428No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT6590.10777606782532755No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT6590.10777606782532755No Hit
GGACATTGGCAGTTATTTAGCCTGGTATCAGCAAAAATCAGGGAAAGCCCCTGACCTCCTACTCTTTGCTGCATC6570.10744897808989406No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA6490.1061406191481602No Hit
GACCAGGGTGGAGATCAAAGGAACTGTGGCTGCACCATCTGTCTTCATCT6310.103196811529259No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC6290.10286972179382552No Hit
TCCTTCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCGGGC6120.10008945904264105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)