FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004684540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015545
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38770.381765455986687No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36180.35626190863034135No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTACTCAGAGCCTCCTG30570.3010206342407279No Hit
CTACACAGCTGGCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA25910.2551339428582682No Hit
CTATTATTGCATGCAAACTACACAGCTGGCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGG24890.2450900747874294No Hit
GTGATGGAAAGACCTATTTACATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGTTCCTCATCTATGAAGTTT24590.24213599594306504No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24490.24115130299494358No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24050.2368186540232092No Hit
GGTCTACTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTACATTGGTACCTGCAGAAGCCAGGCCAGCCTC22770.2242145842872546No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22520.221752851916951No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21980.21643550999709515No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20670.20353603237670415No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20250.199400321994594No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19950.19644624315022968No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19900.19595389667616897No Hit
CCTGAATCCAGTGCAGATATTGTGATGACCCAGAGTCCACTCTCTCTGTC19690.1938860414851139No Hit
CAATAATAGACCCCAACATCCTCAGTCTCCACGCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA19690.1938860414851139No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18990.18699319084826374No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18530.18246360328690506No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17920.1764569763033642No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17800.17527534476561846No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGATATTGTGATGA17670.17399524393306057No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16360.16109576631266956No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA16210.1596187268904874No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA16110.15863403394236592No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16000.15755087169943233No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT15890.15646770945649874No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15600.15361209990694652No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG15470.15233199907438863No Hit
GTTCTCTGGAGTGTCAGACAGGTTCAGTGGCAGCGGGTCAGGGACAGATT15430.15193812189514003No Hit
GCTGTGTAGTTTGCATGCAATAATAGACCCCAACATCCTCAGTCTCCACGCGGCTGATTTTCAGTGTGAAAT15280.15046108247295786No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15220.14987026670408501No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14770.14543914843753847No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGATATTGTGATGACCCAGAGTCCACTCTCT14670.14445445548941702No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14630.14406057831016844No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14580.1435682318361077No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14550.14327282395167126No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14500.14278047747761055No Hit
GGATACCTGAATCCAGTGCAGATATTGTGATGACCCAGAGTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGG14400.1417957845294891No Hit
GTGCTGGGTTCTCCTTGTTGCTATTTTAGAAGGTTTCAAGTGTGAACTGC14360.14140190735024052No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14270.1405156836969312No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13630.1342136488289539No Hit
TTCCATCACTATGCAGGAGGCTCTGAGTAGACCTGCAGGAGATGGAGGCC13530.13322895588083247No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13360.13155497786902598No Hit
GTGTAGGCTCTGGCTTCACGTTCGGAAACTATGAAATGAATTGGGTCCGC13300.13096416210015313No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13210.13007793844684382No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13110.12909324549872236No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13070.12869936831947376No Hit
ACTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTACATTGGTACCT13050.1285024297298495No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13030.1283054911402252No Hit
GCTAATGCTCTGGATACCTGAATCCAGTGCAGATATTGTGATGACCCAGAGTCCACTCTCTCTGTCCGTCACCC12950.12751773678172804No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12860.12663151312841875No Hit
TCTATGAAGTTTCCAATCGGTTCTCTGGAGTGTCAGACAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACA12830.1263361052439823No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12790.12594222806473374No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG12710.12515447370623656No Hit
GTCTGACACTCCAGAGAACCGATTGGAAACTTCATAGATGAGGAACTGTGGAGGCTGGCCTGGCTTCTGCAGGTA12700.12505600441142442No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12590.12397284216849082No Hit
GAGTTGGGGCTGTGCTGGGTTCTCCTTGTTGCTATTTTAGAAGGTTTCAAGTGTGAACTGCAGTTGGTGGAGT12510.12318508780999365No Hit
CTCCTGCATAGTGATGGAAAGACCTATTTACATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGTTCCTCAT12450.12259427204112078No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12420.12229886415668434No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12410.12220039486187219No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12290.12101876332412645No Hit
GTTCCTCATCTATGAAGTTTCCAATCGGTTCTCTGGAGTGTCAGACAGGT12260.12072335543969001No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC12120.11934478531232No Hit
ATAATAAGCAGCCGTGTCCTCGACTCTCAGGTTGTTCATTTGTAGATACA12050.11865550024863496No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12040.11855703095382283No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11970.11786774589013779No Hit
GTGTCAGACAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAA11910.11727693012126494No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGATATTGTGATGACCCAGAGTCCACT11840.11658764505757993No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC11800.11619376787833134No Hit
CCAGAGAACCGATTGGAAACTTCATAGATGAGGAACTGTGGAGGCTGGCC11750.11570142140427062No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11740.11560295210945847No Hit
GAGTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAGGGGTGACGGACAGA11710.11530754422502203No Hit
GCTTATTATTGTGCGAGAGATGGGTTCGGAACGGAGTACGATTTTTGGAA11670.11491366704577345No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11640.11461825916133703No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11600.11422438198208844No Hit
CTCCTGTGTAGGCTCTGGCTTCACGTTCGGAAACTATGAAATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG11530.11353509691840342No Hit
GTCCAGGGGTGACGGACAGAGAGAGTGGACTCTGGGTCATCACAATATCT11490.11314121973915485No Hit
GTGTAGTTTGCATGCAATAATAGACCCCAACATCCTCAGTCTCCACGCGGCTGATTTTCAGTGTGAAATCTGTCC11370.1119595882014091No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTCTC11360.11186111890659695No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC11320.11146724172734837No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11220.1104825487792269No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11070.10900550935704473No Hit
GGTCAGGGACAGATTTCACACTGAAAATCAGCCGCGTGGAGACTGAGGATGTTGGGGTCTATTATTGCATGCAAA11020.10851316288298402No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10450.10290041307869173No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10400.102408066604631No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10360.10201418942538243No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10260.10102949647726098No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10170.10014327282395166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2050.051.6776661
CTTATAC3100.050.709721
CGTATGC3150.049.36479641
TCGTATG3200.048.54751240
TATCTCG3050.047.35085336
CACGAGA3250.047.2851521
GTATGCC3300.047.17029642
CCCACGA3300.046.56870319
ATGCCGT3350.046.5475244
GGTATCA8050.046.2727931
ACTCCTA3350.046.0141331
CGGACTC3350.045.93472328
CGAGACG3350.045.87590823
TGGTATC2250.045.5338442
TATACAC3500.044.8833663
ATCTCCG3500.044.8789410
TCCTATC3300.044.70370533
AGACGGA3450.044.5637325
ACGAGAC3500.043.90979822
ATCTCGT3300.043.79841237
Produced by FastQC (version 0.11.5)