FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004684541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004684541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015545
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38870.3827501489348084No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34830.34296855383070174No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30540.30072522635629145No Hit
CTACACAGCTGGCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA25220.24833956151623018No Hit
CAATAATAGACCCCAACATCCTCAGTCTCCACGCGGCTGATTTTCAGTGTGAAATCTGTCCCTGACCCGCTGCCA24580.2420375266482529No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24310.239378855688325No Hit
CTATTATTGCATGCAAACTACACAGCTGGCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGG23530.23169825069297767No Hit
CCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTACTCAGAGCCTCCTG22870.22519927723537608No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22380.22037428178958096No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22050.21712479506078014No Hit
GTGATGGAAAGACCTATTTACATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGTTCCTCATCTATGAAGTTT21540.21210286102536077No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20650.20333909378707984No Hit
GGTCTACTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTACATTGGTACCTGCAGAAGCCAGGCCAGCCTC19680.19378757219030177No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19050.18758400661713662No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18210.17931258585291643No Hit
GCTGTGTAGTTTGCATGCAATAATAGACCCCAACATCCTCAGTCTCCACGCGGCTGATTTTCAGTGTGAAAT17970.17694932277742492No Hit
CCTGAATCCAGTGCAGATATTGTGATGACCCAGAGTCCACTCTCTCTGTC17930.17655544559817635No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17590.1732074895745634No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17280.1701549414353869No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16640.16385290656740964No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16550.16296668291410032No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16440.1618835206711667No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16120.15873250323717808No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGATATTGTGATGA15980.15735393310980803No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15840.155975362982438No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15750.15508913932912868No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATA15570.15331669202251008No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15450.15213506048476433No Hit
GTGCTGGGTTCTCCTTGTTGCTATTTTAGAAGGTTTCAAGTGTGAACTGC15330.1509534289470186No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15230.14996873599889712No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15140.14908251234558784No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15140.14908251234558784No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG14930.1470146571545328No Hit
GTTCTCTGGAGTGTCAGACAGGTTCAGTGGCAGCGGGTCAGGGACAGATT14820.14593149491159918No Hit
GTGTAGTTTGCATGCAATAATAGACCCCAACATCCTCAGTCTCCACGCGGCTGATTTTCAGTGTGAAATCTGTCC14550.14327282395167126No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGATATTGTGATGACCCAGAGTCCACTCTCT14350.14130343805542836No Hit
GGATACCTGAATCCAGTGCAGATATTGTGATGACCCAGAGTCCACTCTCTCTGTCCGTCACCCCTGGACAGCCGG14270.1405156836969312No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14120.13903864427474905No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13680.13470599530301464No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13640.13431211812376606No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13420.13214579363789888No Hit
GTCTGACACTCCAGAGAACCGATTGGAAACTTCATAGATGAGGAACTGTGGAGGCTGGCCTGGCTTCTGCAGGTA13260.13057028492090456No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13080.12879783761428593No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13050.1285024297298495No Hit
TTCCATCACTATGCAGGAGGCTCTGAGTAGACCTGCAGGAGATGGAGGCC13010.1281085525506009No Hit
GAGTTGGGGCTGTGCTGGGTTCTCCTTGTTGCTATTTTAGAAGGTTTCAAGTGTGAACTGCAGTTGGTGGAGT13010.1281085525506009No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12820.12623763594917015No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12780.12584375876992157No Hit
CTCTCTGTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA12750.12554835088548513No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12670.12476059652698798No Hit
GTGTAGGCTCTGGCTTCACGTTCGGAAACTATGAAATGAATTGGGTCCGC12560.12367743428405437No Hit
GCTTATTATTGTGCGAGAGATGGGTTCGGAACGGAGTACGATTTTTGGAA12470.12279121063074508No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12440.12249580274630863No Hit
CTCCTGTGTAGGCTCTGGCTTCACGTTCGGAAACTATGAAATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG12120.11934478531232No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12110.11924631601750785No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC12010.11826162306938638No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC11990.1180646844797621No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11970.11786774589013779No Hit
GTTCCTCATCTATGAAGTTTCCAATCGGTTCTCTGGAGTGTCAGACAGGT11840.11658764505757993No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11830.11648917576276778No Hit
GTCCAGGGGTGACGGACAGAGAGAGTGGACTCTGGGTCATCACAATATCT11740.11560295210945847No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11700.11520907493020988No Hit
ACTCAGAGCCTCCTGCATAGTGATGGAAAGACCTATTTACATTGGTACCT11600.11422438198208844No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11520.11343662762359127No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11480.1130427504443427No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG11380.11205805749622123No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11350.1117626496117848No Hit
GTCCCTGACCCGCTGCCACTGAACCTGTCTGACACTCCAGAGAACCGATT11200.11028561018960263No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11190.11018714089479048No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11190.11018714089479048No Hit
TCTATGAAGTTTCCAATCGGTTCTCTGGAGTGTCAGACAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACA11130.10959632512591762No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTCTC11060.1089070400622326No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11010.10841469358817188No Hit
GGTCAGGGACAGATTTCACACTGAAAATCAGCCGCGTGGAGACTGAGGATGTTGGGGTCTATTATTGCATGCAAA10950.10782387781929899No Hit
ATAATAAGCAGCCGTGTCCTCGACTCTCAGGTTGTTCATTTGTAGATACA10890.10723306205042613No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10810.10644530769192896No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10800.10634683839711681No Hit
CTCCTGCATAGTGATGGAAAGACCTATTTACATTGGTACCTGCAGAAGCCAGGCCAGCCTCCACAGTTCCTCAT10730.1056575533334318No Hit
GAGTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAGGGGTGACGGACAGA10730.1056575533334318No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGAATCCAGTGCAGATATTGTGATGACCCAGAGTCCACT10700.10536214544899537No Hit
CCAGAGAACCGATTGGAAACTTCATAGATGAGGAACTGTGGAGGCTGGCC10700.10536214544899537No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC10690.10526367615418322No Hit
GTGTCAGACAGGTTCAGTGGCAGCGGGTCAGGGACAGATTTCACACTGAA10690.10526367615418322No Hit
GCTAATGCTCTGGATACCTGAATCCAGTGCAGATATTGTGATGACCCAGAGTCCACTCTCTCTGTCCGTCACCC10630.10467286038531036No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG10550.1038851060268132No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10380.10221112801500673No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA10370.10211265872019457No Hit
GTCGTGTCCCTTGGCCGAAGGTGGCCAGCTGTGTAGTTTGCATGCAATAATAGACCCCAACATCCTCAGTCTCCA10360.10201418942538243No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10160.10004480352913951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)